CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018173
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium/potassium-transporting ATPase subunit alpha-1 
Protein Synonyms/Alias
 Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 
Gene Name
 Atp1a1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
9GKGVGRDKYEPAAVSacetylation[1]
9GKGVGRDKYEPAAVSubiquitination[2]
21AVSEHGDKKGKKAKKacetylation[1]
21AVSEHGDKKGKKAKKubiquitination[2]
36ERDMDELKKEVSMDDubiquitination[2]
37RDMDELKKEVSMDDHubiquitination[2]
45EVSMDDHKLSLDELHubiquitination[2]
54SLDELHRKYGTDLSRubiquitination[2]
156YQEAKSSKIMESFKNubiquitination[2]
162SKIMESFKNMVPQQAubiquitination[2]
177LVIRNGEKMSINAEDubiquitination[2]
194VGDLVEVKGGDRIPAubiquitination[2]
212IISANGCKVDNSSLTubiquitination[2]
354TAKRMARKNCLVKNLubiquitination[2]
359ARKNCLVKNLEAVETubiquitination[2]
377TSTICSDKTGTLTQNubiquitination[2]
413QSGVSFDKTSATWFAubiquitination[2]
444QENLPILKRAVAGDAubiquitination[2]
476EMREKYSKIVEIPFNubiquitination[2]
487IPFNSTNKYQLSIHKacetylation[3]
487IPFNSTNKYQLSIHKubiquitination[2]
494KYQLSIHKNPNASEPacetylation[4]
494KYQLSIHKNPNASEPubiquitination[2]
502NPNASEPKHLLVMKGubiquitination[2]
508PKHLLVMKGAPERILacetylation[4]
508PKHLLVMKGAPERILubiquitination[2]
526SSILLHGKEQPLDEEubiquitination[2]
535QPLDEELKDAFQNAYacetylation[4]
605AVPDAVGKCRSAGIKubiquitination[2]
625GDHPITAKAIAKGVGubiquitination[2]
629ITAKAIAKGVGIISEubiquitination[2]
661QVNPRDAKACVVHGSacetylation[1]
661QVNPRDAKACVVHGSsuccinylation[1]
661QVNPRDAKACVVHGSubiquitination[2]
671VVHGSDLKDMTSEELubiquitination[2]
698ARTSPQQKLIIVEGCubiquitination[2]
726VNDSPALKKADIGVAubiquitination[2]
727NDSPALKKADIGVAMubiquitination[2]
773RLIFDNLKKSIAYTLubiquitination[2]
840KRQPRNPKTDKLVNEubiquitination[2]
843PRNPKTDKLVNERLIacetylation[5]
843PRNPKTDKLVNERLIubiquitination[2]
950SVFQQGMKNKILIFGubiquitination[2]
1019RPGGWVEKETYY***ubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). 
Sequence Annotation
 REGION 82 84 Phosphoinositide-3 kinase binding (By
 ACT_SITE 376 376 4-aspartylphosphate intermediate (By
 METAL 717 717 Magnesium (By similarity).
 METAL 721 721 Magnesium (By similarity).
 BINDING 487 487 ATP (By similarity).
 MOD_RES 10 10 Phosphotyrosine (By similarity).
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 47 47 Phosphoserine.
 MOD_RES 217 217 Phosphoserine.
 MOD_RES 219 219 Phosphothreonine.
 MOD_RES 228 228 Phosphoserine.
 MOD_RES 260 260 Phosphotyrosine.
 MOD_RES 452 452 Phosphoserine.
 MOD_RES 542 542 Phosphotyrosine (By similarity).
 MOD_RES 943 943 Phosphoserine; by PKA (By similarity).  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Sodium; Sodium transport; Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1023 AA 
Protein Sequence
MGKGVGRDKY EPAAVSEHGD KKGKKAKKER DMDELKKEVS MDDHKLSLDE LHRKYGTDLS 60
RGLTPARAAE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF SMLLWIGAIL CFLAYGIRSA 120
TEEEPPNDDL YLGVVLSAVV IITGCFSYYQ EAKSSKIMES FKNMVPQQAL VIRNGEKMSI 180
NAEDVVVGDL VEVKGGDRIP ADLRIISANG CKVDNSSLTG ESEPQTRSPD FTNENPLETR 240
NIAFFSTNCV EGTARGIVVY TGDRTVMGRI ATLASGLEGG QTPIAEEIEH FIHLITGVAV 300
FLGVSFFILS LILEYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR MARKNCLVKN 360
LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA DTTENQSGVS FDKTSATWFA 420
LSRIAGLCNR AVFQANQENL PILKRAVAGD ASESALLKCI EVCCGSVMEM REKYSKIVEI 480
PFNSTNKYQL SIHKNPNASE PKHLLVMKGA PERILDRCSS ILLHGKEQPL DEELKDAFQN 540
AYLELGGLGE RVLGFCHLLL PDEQFPEGFQ FDTDDVNFPV DNLCFVGLIS MIDPPRAAVP 600
DAVGKCRSAG IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI PVNQVNPRDA 660
KACVVHGSDL KDMTSEELDD ILRYHTEIVF ARTSPQQKLI IVEGCQRQGA IVAVTGDGVN 720
DSPALKKADI GVAMGIVGSD VSKQAADMIL LDDNFASIVT GVEEGRLIFD NLKKSIAYTL 780
TSNIPEITPF LIFIIANIPL PLGTVTILCI DLGTDMVPAI SLAYEQAESD IMKRQPRNPK 840
TDKLVNERLI SMAYGQIGMI QALGGFFTYF VILAENGFLP FHLLGIRETW DDRWVNDVED 900
SYGQQWTYEQ RKIVEFTCHT AFFVSIVVVQ WADLVICKTR RNSVFQQGMK NKILIFGLFE 960
ETALAAFLSY CPGMGAALRM YPLKPTWWFC AFPYSLLIFV YDEVRKLIIR RRPGGWVEKE 1020
TYY 1023 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IDA:MGI.
 GO:0005901; C:caveola; IEA:Compara.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0005768; C:endosome; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0016021; C:integral to membrane; ISS:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005890; C:sodium:potassium-exchanging ATPase complex; IEA:Compara.
 GO:0030315; C:T-tubule; IDA:BHF-UCL.
 GO:0003869; F:4-nitrophenylphosphatase activity; IMP:MGI.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030955; F:potassium ion binding; IEA:Compara.
 GO:0031402; F:sodium ion binding; IEA:Compara.
 GO:0005391; F:sodium:potassium-exchanging ATPase activity; IMP:MGI.
 GO:0006754; P:ATP biosynthetic process; IEA:InterPro.
 GO:0071260; P:cellular response to mechanical stimulus; IEA:Compara.
 GO:0060081; P:membrane hyperpolarization; IEA:Compara.
 GO:0031947; P:negative regulation of glucocorticoid biosynthetic process; IMP:MGI.
 GO:0045822; P:negative regulation of heart contraction; IMP:MGI.
 GO:0045823; P:positive regulation of heart contraction; IMP:MGI.
 GO:0045989; P:positive regulation of striated muscle contraction; IMP:MGI.
 GO:0010107; P:potassium ion import; IEA:Compara.
 GO:0008217; P:regulation of blood pressure; IGI:MGI.
 GO:0086004; P:regulation of cardiac muscle cell contraction; IEA:Compara.
 GO:0002028; P:regulation of sodium ion transport; ISS:UniProtKB.
 GO:0002026; P:regulation of the force of heart contraction; IMP:MGI.
 GO:0042493; P:response to drug; IMP:MGI. 
Interpro
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR005775; ATPase_P-typ_Na/K_IIC.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.