CPLM-007833

Genbank Nucleotide ID

60S ribosomal protein L28

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
19	NCSSFLIKRNKQTYS	ubiquitination	[1, 2, 3, 4]
22	SFLIKRNKQTYSTEP	ubiquitination	[1, 2, 3, 4]
33	STEPNNLKARNSFRY	ubiquitination	[1, 2, 3, 4, 5]
47	YNGLIHRKTVGVEPA	ubiquitination	[1, 2, 3, 4, 6]
58	VEPAADGKGVVVVIK	ubiquitination	[1, 2, 3, 4, 6]
65	KGVVVVIKRRSGQRK	ubiquitination	[1, 3, 4, 6]
72	KRRSGQRKPATSYVR	ubiquitination	[3, 7]
84	YVRTTINKNARATLS	ubiquitination	[2]
101	RHMIRKNKYRPDLRM	acetylation	[8]
101	RHMIRKNKYRPDLRM	ubiquitination	[1, 3, 4, 6]
122	SAILRSQKPVMVKRK	ubiquitination	[2, 3, 4]
127	SQKPVMVKRKRTRPT	ubiquitination	[4]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

MOD_RES 2 2 N-acetylserine.
MOD_RES 115 115 Phosphoserine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0022625; C:cytosolic large ribosomal subunit; IDA:UniProtKB.
GO:0003723; F:RNA binding; TAS:ProtInc.
GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB.
GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
GO:0006414; P:translational elongation; TAS:Reactome.
GO:0006413; P:translational initiation; TAS:Reactome.
GO:0006415; P:translational termination; TAS:Reactome.
GO:0019083; P:viral transcription; TAS:Reactome.

IPR002672; Ribosomal_L28e.