UniProt Accession

 SKA3_HUMAN; Q8IX90; A2A330; A2A331; B2RBY2; Q5VZV5; Q86WR2; Q8NBG1; Q96D22 

Genbank Protein ID

 AF361358; AK090584; AK314864; AL158032; AL158032; AL158032; CH471075; CH471075; CH471075; BC013418; BC048988 

Genbank Nucleotide ID

 AAO13796.1; BAC03484.1; BAG37379.1; CAH69950.1; CAM13059.1; CAM13060.1; EAX08293.1; EAX08294.1; EAX08295.1; AAH13418.1; AAH48988.1 

Protein Name

 Spindle and kinetochore-associated protein 3 

Protein Synonyms/Alias

  

Gene Name

 SKA3 

Gene Synonyms/Alias

 C13orf3; RAMA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	PIRSFCGKLRSLAST	ubiquitination	[1]
77	QEGIDFIKATKVLME	ubiquitination	[1]
99	KIREYFQKYGYSPRV	ubiquitination	[1, 2]
151	ASSCISEKSPRSPQL	ubiquitination	[1]
183	PQAVNNYKEEPVIVT	ubiquitination	[1]
199	PTKQSLVKVLKTPKC	ubiquitination	[1, 2]
209	KTPKCALKMDDFECV	ubiquitination	[1]
247	LKNARNNKSEEAIDT	ubiquitination	[1]
295	TFCTPGLKIPSTKNS	ubiquitination	[1]
300	GLKIPSTKNSIALVS	ubiquitination	[1]
314	STNYPLSKTNSSSND	ubiquitination	[1]
366	PTPPEVTKIPEDILQ	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. 

Sequence Annotation

 MOD_RES 34 34 Phosphoserine.
 MOD_RES 79 79 Phosphothreonine (By similarity).
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 152 152 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 265 265 Phosphothreonine.
 MOD_RES 267 267 Phosphoserine.
 MOD_RES 318 318 Phosphoserine.
 MOD_RES 384 384 Phosphothreonine.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 412 AA 

Protein Sequence

Gene Ontology

 GO:0000940; C:condensed chromosome outer kinetochore; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005876; C:spindle microtubule; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0007067; P:mitosis; IMP:UniProtKB.
 GO:0031110; P:regulation of microtubule polymerization or depolymerization; IDA:UniProtKB. 

Interpro

  

Pfam

  

SMART

  

PROSITE

  

PRINTS