CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023699
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Axin-2 
Protein Synonyms/Alias
 Axin-like protein; Axil; Axis inhibition protein 2; Conductin 
Gene Name
 AXIN2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
528HHAVPKTKEEIEAEAubiquitination[1]
792SLTLGHFKEQLSKKGacetylation[2]
797HFKEQLSKKGNYRYYacetylation[2]
806GNYRYYFKKASDEFAacetylation[2]
838YEGRILGKVERID**ubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta- catenin and APC by GSK3B (By similarity). 
Sequence Annotation
 DOMAIN 81 200 RGS.
 DOMAIN 761 843 DIX.
 REGION 327 413 Interaction with GSK3B (By similarity).
 REGION 413 476 Interaction with beta-catenin (By
 MOTIF 21 30 Tankyrase-binding motif.
 MOD_RES 460 460 Phosphotyrosine (By similarity).
 MOD_RES 464 464 Phosphoserine (By similarity).  
Keyword
 ADP-ribosylation; Complete proteome; Cytoplasm; Developmental protein; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 843 AA 
Protein Sequence
MSSAMLVTCL PDPSSSFRED APRPPVPGEE GETPPCQPGV GKGQVTKPMS VSSNTRRNED 60
GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE REKCVDTLDF WFACNGFRQM 120
NLKDTKTLRV AKAIYKRYIE NNSIVSKQLK PATKTYIRDG IKKQQIDSIM FDQAQTEIQS 180
VMEENAYQMF LTSDIYLEYV RSGGENTAYM SNGGLGSLKV VCGYLPTLNE EEEWTCADFK 240
CKLSPTVVGL SSKTLRATAS VRSTETVDSG YRSFKRSDPV NPYHIGSGYV FAPATSANDS 300
EISSDALTDD SMSMTDSSVD GIPPYRVGSK KQLQREMHRS VKANGQVSLP HFPRTHRLPK 360
EMTPVEPATF AAELISRLEK LKLELESRHS LEERLQQIRE DEEREGSELT LNSREGAPTQ 420
HPLSLLPSGS YEEDPQTILD DHLSRVLKTP GCQSPGVGRY SPRSRSPDHH HHHHSQYHSL 480
LPPGGKLPPA AASPGACPLL GGKGFVTKQT TKHVHHHYIH HHAVPKTKEE IEAEATQRVH 540
CFCPGGSEYY CYSKCKSHSK APETMPSEQF GGSRGSTLPK RNGKGTEPGL ALPAREGGAP 600
GGAGALQLPR EEGDRSQDVW QWMLESERQS KPKPHSAQST KKAYPLESAR SSPGERASRH 660
HLWGGNSGHP RTTPRAHLFT QDPAMPPLTP PNTLAQLEEA CRRLAEVSKP PKQRCCVASQ 720
QRDRNHSATV QTGATPFSNP SLAPEDHKEP KKLAGVHALQ ASELVVTYFF CGEEIPYRRM 780
LKAQSLTLGH FKEQLSKKGN YRYYFKKASD EFACGAVFEE IWEDETVLPM YEGRILGKVE 840
RID 843 
Gene Ontology
 GO:0030877; C:beta-catenin destruction complex; NAS:BHF-UCL.
 GO:0005938; C:cell cortex; IBA:RefGenome.
 GO:0005813; C:centrosome; IDA:BHF-UCL.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IBA:RefGenome.
 GO:0005881; C:cytoplasmic microtubule; IBA:RefGenome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IBA:RefGenome.
 GO:0014069; C:postsynaptic density; IBA:RefGenome.
 GO:0070016; F:armadillo repeat domain binding; IBA:RefGenome.
 GO:0008013; F:beta-catenin binding; NAS:BHF-UCL.
 GO:0005096; F:GTPase activator activity; IBA:RefGenome.
 GO:0019901; F:protein kinase binding; IBA:RefGenome.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0030282; P:bone mineralization; IEA:Compara.
 GO:0008219; P:cell death; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0034613; P:cellular protein localization; IDA:BHF-UCL.
 GO:0071407; P:cellular response to organic cyclic compound; IBA:RefGenome.
 GO:0003413; P:chondrocyte differentiation involved in endochondral bone morphogenesis; IEA:Compara.
 GO:0009950; P:dorsal/ventral axis specification; IBA:RefGenome.
 GO:0001957; P:intramembranous ossification; IBA:RefGenome.
 GO:0043570; P:maintenance of DNA repeat elements; IMP:BHF-UCL.
 GO:0048255; P:mRNA stabilization; IMP:BHF-UCL.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IDA:BHF-UCL.
 GO:0035414; P:negative regulation of catenin import into nucleus; IMP:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; IMP:BHF-UCL.
 GO:0045668; P:negative regulation of osteoblast differentiation; IBA:RefGenome.
 GO:2000054; P:negative regulation of Wnt receptor signaling pathway involved in dorsal/ventral axis specification; IEA:Compara.
 GO:0042476; P:odontogenesis; IMP:BHF-UCL.
 GO:0010942; P:positive regulation of cell death; IMP:BHF-UCL.
 GO:0010718; P:positive regulation of epithelial to mesenchymal transition; IMP:BHF-UCL.
 GO:0045860; P:positive regulation of protein kinase activity; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IMP:BHF-UCL.
 GO:0070602; P:regulation of centromeric sister chromatid cohesion; IMP:BHF-UCL.
 GO:0061181; P:regulation of chondrocyte development; IEA:Compara.
 GO:0032423; P:regulation of mismatch repair; IMP:BHF-UCL.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0090244; P:Wnt receptor signaling pathway involved in somitogenesis; IBA:RefGenome. 
Interpro
 IPR014936; Axin_b-cat-bd.
 IPR001158; DIX.
 IPR000342; Regulat_G_prot_signal.
 IPR024066; Regulat_G_prot_signal_dom1.
 IPR016137; Regulat_G_prot_signal_superfam. 
Pfam
 PF08833; Axin_b-cat_bind
 PF00778; DIX
 PF00615; RGS 
SMART
 SM00021; DAX
 SM00315; RGS 
PROSITE
 PS50841; DIX
 PS50132; RGS 
PRINTS
 PR01301; RGSPROTEIN.