CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004084
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Catalase-peroxidase 
Protein Synonyms/Alias
 CP; Hydroperoxidase I; HPI; Peroxidase/catalase 
Gene Name
 katG 
Gene Synonyms/Alias
 b3942; JW3914 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
64GEDFDYRKEFSKLDYacetylation[1]
68DYRKEFSKLDYYGLKacetylation[1]
75KLDYYGLKKDLKALLacetylation[1]
141PDNVSLDKARRLLWPacetylation[1]
367PDPFDPSKKRKPTMLacetylation[1]
370FDPSKKRKPTMLVTDacetylation[2]
388RFDPEFEKISRRFLNacetylation[1]
429YIGPEVPKEDLIWQDacetylation[1]
515RALPVLEKIQKESGKacetylation[1]
627GANFDGSKNGVFTDRacetylation[1]
654LDMRYEWKATDESKEacetylation[1]
660WKATDESKELFEGRDacetylation[1, 2]
674DRETGEVKFTASRADacetylation[1]
708DAHEKFVKDFVAAWVacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Bifunctional enzyme with both catalase and broad- spectrum peroxidase activity. Displays also NADH oxidase, INH lyase and isonicotinoyl-NAD synthase activity. 
Sequence Annotation
 ACT_SITE 106 106 Proton acceptor (By similarity).
 METAL 267 267 Iron (heme axial ligand).
 CROSSLNK 105 226 Tryptophyl-tyrosyl-methioninium (Trp-Tyr)
 CROSSLNK 226 252 Tryptophyl-tyrosyl-methioninium (Tyr-Met)  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Heme; Hydrogen peroxide; Iron; Metal-binding; Oxidoreductase; Peroxidase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 726 AA 
Protein Sequence
MSTSDDIHNT TATGKCPFHQ GGHDQSAGAG TTTRDWWPNQ LRVDLLNQHS NRSNPLGEDF 60
DYRKEFSKLD YYGLKKDLKA LLTESQPWWP ADWGSYAGLF IRMAWHGAGT YRSIDGRGGA 120
GRGQQRFAPL NSWPDNVSLD KARRLLWPIK QKYGQKISWA DLFILAGNVA LENSGFRTFG 180
FGAGREDVWE PDLDVNWGDE KAWLTHRHPE ALAKAPLGAT EMGLIYVNPE GPDHSGEPLS 240
AAAAIRATFG NMGMNDEETV ALIAGGHTLG KTHGAGPTSN VGPDPEAAPI EEQGLGWAST 300
YGSGVGADAI TSGLEVVWTQ TPTQWSNYFF ENLFKYEWVQ TRSPAGAIQF EAVDAPEIIP 360
DPFDPSKKRK PTMLVTDLTL RFDPEFEKIS RRFLNDPQAF NEAFARAWFK LTHRDMGPKS 420
RYIGPEVPKE DLIWQDPLPQ PIYNPTEQDI IDLKFAIADS GLSVSELVSV AWASASTFRG 480
GDKRGGANGA RLALMPQRDW DVNAAAVRAL PVLEKIQKES GKASLADIIV LAGVVGVEKA 540
ASAAGLSIHV PFAPGRVDAR QDQTDIEMFE LLEPIADGFR NYRARLDVST TESLLIDKAQ 600
QLTLTAPEMT ALVGGMRVLG ANFDGSKNGV FTDRVGVLSN DFFVNLLDMR YEWKATDESK 660
ELFEGRDRET GEVKFTASRA DLVFGSNSVL RAVAEVYASS DAHEKFVKDF VAAWVKVMNL 720
DRFDLL 726 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004096; F:catalase activity; IDA:EcoCyc.
 GO:0020037; F:heme binding; IDA:EcoCyc.
 GO:0042802; F:identical protein binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042744; P:hydrogen peroxide catabolic process; IMP:EcoCyc. 
Interpro
 IPR000763; Catalase_peroxidase.
 IPR010255; Haem_peroxidase.
 IPR002016; Haem_peroxidase_pln/fun/bac.
 IPR019794; Peroxidases_AS.
 IPR019793; Peroxidases_heam-ligand_BS. 
Pfam
 PF00141; peroxidase 
SMART
  
PROSITE
 PS00435; PEROXIDASE_1
 PS00436; PEROXIDASE_2
 PS50873; PEROXIDASE_4 
PRINTS
 PR00460; BPEROXIDASE.
 PR00458; PEROXIDASE.