CPLM-023250

Genbank Nucleotide ID

FAS-associated factor 1

Protein Synonyms/Alias

hFAF1; UBX domain-containing protein 12; UBX domain-containing protein 3A

UBXD12; UBXN3A; CGI-03

Homo sapiens (Human)

Position	Peptide	Type	References
139	ELQIPVSKMLLKGWK	ubiquitination	[1, 2, 3]
143	PVSKMLLKGWKTGDV	ubiquitination	[1, 3]
146	KMLLKGWKTGDVEDS	ubiquitination	[1, 2, 3, 4, 5]
157	VEDSTVLKSLHLPKN	ubiquitination	[1, 2, 3, 4, 5, 6]
221	SSTIQEVKRNVYDLT	ubiquitination	[1, 2, 3, 5]
444	AQTIRTQKTDQFPLF	ubiquitination	[2, 3]
457	LFLIIMGKRSSNEVL	ubiquitination	[2, 6, 7]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
[7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Potentiates but cannot initiate FAS-induced apoptosis.

DOMAIN 1 57 UBA.
DOMAIN 569 646 UBX.
MOD_RES 270 270 Phosphoserine (By similarity).
MOD_RES 320 320 Phosphoserine.
MOD_RES 580 580 Phosphothreonine (By similarity).

3D-structure; Alternative splicing; Apoptosis; Complete proteome; Nucleus; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031265; C:CD95 death-inducing signaling complex; NAS:UniProtKB.
GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:BHF-UCL.
GO:0005829; C:cytosol; IDA:UniProtKB.
GO:0005635; C:nuclear envelope; IEA:Compara.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0031072; F:heat shock protein binding; IDA:UniProtKB.
GO:0019901; F:protein kinase binding; IDA:UniProtKB.
GO:0019887; F:protein kinase regulator activity; NAS:UniProtKB.
GO:0043130; F:ubiquitin binding; IDA:BHF-UCL.
GO:0031625; F:ubiquitin protein ligase binding; IDA:BHF-UCL.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0008219; P:cell death; IMP:UniProtKB.
GO:0007253; P:cytoplasmic sequestering of NF-kappaB; IMP:UniProtKB.
GO:0043065; P:positive regulation of apoptotic process; NAS:UniProtKB.
GO:0031334; P:positive regulation of protein complex assembly; IMP:UniProtKB.
GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; NAS:UniProtKB.
GO:0030155; P:regulation of cell adhesion; IEA:Compara.
GO:0042176; P:regulation of protein catabolic process; IMP:UniProtKB.

IPR006577; UAS.
IPR001012; UBX.

SM00594; UAS
SM00166; UBX

PS50030; UBA
PS50033; UBX