CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008540
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucokinase 
Protein Synonyms/Alias
 Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D 
Gene Name
 Gck 
Gene Synonyms/Alias
 Gk 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
46KEMDRGLKLETHQEAubiquitination[1]
56THQEASVKMLPTYVRubiquitination[1]
102EAGQWSVKTKHQMYSubiquitination[1]
161VRHEDIDKGILLNWTubiquitination[1]
169GILLNWTKGFKASGAubiquitination[1]
172LNWTKGFKASGAEGNubiquitination[1]
291PGQQLYEKIIGGKYMubiquitination[1]
296YEKIIGGKYMGELVRubiquitination[1]
414GVDGSVYKLHPSFKEubiquitination[1]
420YKLHPSFKERFHASVubiquitination[1]
458LVSAVACKKACMLGQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. 
Sequence Annotation
 NP_BIND 78 83 ATP (Potential).
 NP_BIND 295 296 ATP (By similarity).
 NP_BIND 332 336 ATP (By similarity).
 NP_BIND 411 415 ATP (By similarity).
 REGION 151 152 Substrate binding (By similarity).
 REGION 168 169 Substrate binding (By similarity).
 REGION 204 205 Substrate binding (By similarity).
 BINDING 104 104 ATP (Potential).
 BINDING 228 228 ATP (By similarity).
 BINDING 231 231 Substrate (By similarity).
 BINDING 256 256 Substrate (By similarity).
 BINDING 290 290 Substrate (By similarity).  
Keyword
 Alternative promoter usage; Alternative splicing; ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 465 AA 
Protein Sequence
MLDDRARMEA TKKEKVEQIL AEFQLQEEDL KKVMSRMQKE MDRGLKLETH QEASVKMLPT 60
YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEAGQWS VKTKHQMYSI PEDAMTGTAE 120
MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN 180
IVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDRQC EVGMIVGTGC NACYMEEMQN 240
VELVEGDEGR MCVNTEWGAF GNSGELDEFL LEYDRMVDES SVNPGQQLYE KIIGGKYMGE 300
LVRLVLLKLV EENLLFHGEA SEQLRTRGAF ETRFVSQVES DSGDRRQILN ILSTLGLRPS 360
VADCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK 420
ERFHASVRRL TPNCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ 465 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:Compara.
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0030141; C:secretory granule; IEA:Compara.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0004340; F:glucokinase activity; ISS:UniProtKB.
 GO:0005536; F:glucose binding; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0070509; P:calcium ion import; IMP:UniProtKB.
 GO:0001678; P:cellular glucose homeostasis; IMP:MGI.
 GO:0042149; P:cellular response to glucose starvation; IEA:Compara.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0044320; P:cellular response to leptin stimulus; IEA:Compara.
 GO:0051594; P:detection of glucose; ISS:UniProtKB.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IEA:Compara.
 GO:0031018; P:endocrine pancreas development; TAS:Reactome.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IEA:Compara.
 GO:0005978; P:glycogen biosynthetic process; IEA:Compara.
 GO:0006096; P:glycolysis; IBA:RefGenome.
 GO:0006739; P:NADP metabolic process; IMP:MGI.
 GO:0032811; P:negative regulation of epinephrine secretion; IEA:Compara.
 GO:0045721; P:negative regulation of gluconeogenesis; ISS:UniProtKB.
 GO:0045725; P:positive regulation of glycogen biosynthetic process; ISS:UniProtKB.
 GO:0045821; P:positive regulation of glycolysis; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IMP:UniProtKB.
 GO:0042327; P:positive regulation of phosphorylation; IEA:Compara.
 GO:0043266; P:regulation of potassium ion transport; IMP:MGI.
 GO:0019932; P:second-messenger-mediated signaling; IEA:Compara. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.