UniProt Accession

 SYP_MYCTU; O05814; L0TB17 

Genbank Protein ID

 AE000516; AL123456 

Genbank Nucleotide ID

 AAK47237.1; CCP45646.1 

Protein Name

 Proline--tRNA ligase 

Protein Synonyms/Alias

 Prolyl-tRNA synthetase; ProRS 

Gene Name

 proS 

Gene Synonyms/Alias

 Rv2845c; MT2911; MTCY24A1.12 

Created Date

 July 27, 2013 

Organism

 Mycobacterium tuberculosis 

NCBI Taxa ID

 1773 

Lysine Modification

Position	Peptide	Type	References
173	DIDAAGLKAAYHAHR	pupylation	[1]

Reference

 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 

Functional Description

 Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity). 

Sequence Annotation

 CROSSLNK 173 173 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 582 AA 

Protein Sequence

Gene Ontology

 GO:0005618; C:cell wall; IDA:MTBBASE.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:MTBBASE.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004827; F:proline-tRNA ligase activity; IEA:EC.
 GO:0040007; P:growth; IMP:MTBBASE.
 GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 

Interpro

 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR002316; Pro-tRNA-ligase_IIa.
 IPR004500; Pro-tRNA-synth_IIa_bac-type.
 IPR023717; Pro-tRNA-Synthase_IIa_type1.
 IPR007214; YbaK/aa-tRNA-synth-assoc-dom. 

Pfam

 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b
 PF04073; YbaK 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS

 PR01046; TRNASYNTHPRO.