UniProt Accession

 NASP_HUMAN; P49321; A8K6H2; B4DQP3; D3DQ07; F5H3J2; Q53GW5; Q5T622; Q5T625; Q96A69; Q9BTW2 

Genbank Protein ID

 M97856; AK056161; AK291637; AK298893; AK222816; AL355480; AL355480; CH471059; CH471059; BC003113; BC053608; BC010105; BC011580 

Genbank Nucleotide ID

 AAA36027.1; BAF84326.1; BAG61005.1; BAD96536.1; CAI22462.1; CAI22465.1; EAX06968.1; EAX06969.1; AAH03113.1; AAH53608.1; AAH10105.1; AAH11580.1 

Protein Name

 Nuclear autoantigenic sperm protein 

Protein Synonyms/Alias

 NASP 

Gene Name

 NASP 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	DVDSEAKKLLGLGQK	ubiquitination	[1]
54	KLLGLGQKHLVMGDI	ubiquitination	[1]
78	AASLLGKKYGETANE	ubiquitination	[1]
95	EAFFFYGKSLLELAR	ubiquitination	[1, 2, 3]
169	TEDKSLAKPETDKEQ	ubiquitination	[3]
428	VPSQEETKLSVEESE	ubiquitination	[3]
444	AGDGVDTKVAQGATE	ubiquitination	[3]
537	IFKRQETKEAQLYAA	ubiquitination	[1, 3, 4]
645	KKEIEELKELLPEIR	ubiquitination	[1, 3]
659	REKIEDAKESQRSGN	ubiquitination	[3]
673	NVAELALKATLVESS	ubiquitination	[3]
700	VSMIASRKPTDGASS	ubiquitination	[1, 2, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity). 

Sequence Annotation

 REPEAT 43 76 TPR 1.
 REPEAT 542 575 TPR 2.
 REPEAT 584 617 TPR 3.
 REGION 116 127 Histone-binding.
 REGION 211 244 Histone-binding.
 REGION 469 512 Histone-binding.
 MOTIF 716 722 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 33 33 N6-acetyllysine.
 MOD_RES 123 123 Phosphothreonine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 390 390 Phosphothreonine.
 MOD_RES 408 408 Phosphoserine.
 MOD_RES 421 421 Phosphoserine.
 MOD_RES 451 451 Phosphoserine.
 MOD_RES 464 464 Phosphothreonine.
 MOD_RES 477 477 Phosphothreonine.
 MOD_RES 480 480 Phosphoserine.
 MOD_RES 490 490 Phosphothreonine.
 MOD_RES 497 497 Phosphoserine.
 MOD_RES 503 503 Phosphoserine.
 MOD_RES 683 683 Phosphothreonine.
 MOD_RES 726 726 Phosphoserine.
 MOD_RES 751 751 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; TPR repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 788 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0051879; F:Hsp90 protein binding; ISS:UniProtKB.
 GO:0001824; P:blastocyst development; ISS:UniProtKB.
 GO:0007049; P:cell cycle; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; ISS:UniProtKB.
 GO:0006260; P:DNA replication; ISS:UniProtKB.
 GO:0043486; P:histone exchange; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR019544; Tetratricopeptide_SHNi-TPR_dom.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF10516; SHNi-TPR
 PF00515; TPR_1 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS