CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007255
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional regulatory protein DOT6 
Protein Synonyms/Alias
 Disrupter of telomere silencing protein 6; PAC-binding factor 2 
Gene Name
 DOT6 
Gene Synonyms/Alias
 PBF2; YER088C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
39SSSTAMSKNEAQESSubiquitination[1]
70ASSKNSSKNPSSWDPacetylation[2]
139NTYTGPLKITHGDETacetylation[2]
156AQQKPSKKVEENVLTubiquitination[3]
606MNRTPNSKNPQDIALacetylation[2]
606MNRTPNSKNPQDIALubiquitination[3]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the RPD3 histone deacetylase complex RPD3C(L) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. DOT6 binds to sequences containing the core CGATG, which resembles the PAC (Polymerase A and C) motif. 
Sequence Annotation
 DOMAIN 67 121 HTH myb-type.
 DNA_BIND 94 117 H-T-H motif (By similarity).
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 487 487 Phosphoserine.
 MOD_RES 489 489 Phosphothreonine.
 MOD_RES 491 491 Phosphoserine.  
Keyword
 Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 670 AA 
Protein Sequence
MSISTSLNSA SIHLSSMDTH PQLHSLTRQP HSSSTAMSKN EAQESSPSLP ASSSSSTSAS 60
ASASSKNSSK NPSSWDPQDD LLLRHLKEVK KMGWKDISQY FPNRTPNACQ FRWRRLKSGN 120
LKSNKTALID INTYTGPLKI THGDETANAQ QKPSKKVEEN VLTEDTAEFT TTSSIPIPSR 180
KTSLPSFHAS MSFSQSPSNV TPTTIVSNAA SSMPFAPPTL PAALPHHPHQ HLHHHPHHKT 240
LKPRSNSHSF TNSLNQDPIV RSNDEEKYGF IPKVFVRSRR SSFAYPQQVA ITTTPSSPNS 300
SHVLLSSKSR RGSLANWSRR SSFNVSSNNT SRRSSMILAP NSVSNIFNVN NSGSNTASTS 360
NTNSRRESVI KKEFQQRLNN LSNSGGPTSN NGPIFPNSYT FMDLPHSSSV SSSSTLHKSK 420
RGSFSGHSMK SSCNPTNLWS KDEDALLMEN KKRNLSVMEL SILLPQRTEV EIQWRLNALS 480
SDADMLSPTH SPQKTLSKKT CPRMFKSGST TDDDKGSDKE DVMGDGSNDD DEDNVDPLHR 540
AKQSSNKTVF SSSSSNISSK DVSPDPIFSP DPADDSSNTS DAGSRCTITS DTSSSAATMN 600
RTPNSKNPQD IALLNNFRSE AITPRPKPSS TTTSITTETT NNMINHSSST TTTTNNSPLP 660
SINTIFKDML 670 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0033698; C:Rpd3L complex; IDA:SGD.
 GO:0070210; C:Rpd3L-Expanded complex; IDA:SGD.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0000183; P:chromatin silencing at rDNA; IGI:SGD.
 GO:0006348; P:chromatin silencing at telomere; IGI:SGD.
 GO:0030447; P:filamentous growth; IGI:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0009826; P:unidimensional cell growth; IMP:SGD. 
Interpro
 IPR009057; Homeodomain-like.
 IPR017930; Myb_dom.
 IPR001005; SANT/Myb. 
Pfam
 PF00249; Myb_DNA-binding 
SMART
 SM00717; SANT 
PROSITE
 PS51294; HTH_MYB 
PRINTS