UniProt Accession

 RIDA_ECOLI; P0AF93; P39330; P76806; Q2M664 

Genbank Protein ID

 U14003; U00096; AP009048 

Genbank Nucleotide ID

 AAA97140.1; AAC77200.2; BAE78242.1 

Protein Name

 Enamine/imine deaminase 

Protein Synonyms/Alias

  

Gene Name

 ridA 

Gene Synonyms/Alias

 yjgF; b4243; JW5755 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
38	GQIPVNPKTGEVPAD	acetylation	[1]
58	RQSLDNVKAIVEAAG	acetylation	[1, 2]
67	IVEAAGLKVGDIVKT	acetylation	[1]
73	LKVGDIVKTTVFVKD	acetylation	[1]
115	VEVARLPKDVKIEIE	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities. Involved in the isoleucine biosynthesis (By similarity). 

Sequence Annotation

 BINDING 105 105 Substrate (Potential).  

Keyword

 3D-structure; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Detoxification; Direct protein sequencing; Hydrolase; Isoleucine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 128 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0019239; F:deaminase activity; ISS:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW. 

Interpro

 IPR013813; Endoribo_LPSP/chorism_mut-like.
 IPR006056; YjgF-like.
 IPR019897; YjgF-like_CS.
 IPR006175; YjgF/Yer057p/UK114. 

Pfam

 PF01042; Ribonuc_L-PSP 

SMART

  

PROSITE

 PS01094; UPF0076 

PRINTS