CPLM-001877

Genbank Nucleotide ID

Protein Synonyms/Alias

K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas, N-terminally processed

Homo sapiens (Human)

Position	Peptide	Type	References
117	PMVLVGNKCDLPSRT	ubiquitination	[1, 2]
128	PSRTVDTKQAQDLAR	ubiquitination	[1, 2, 3, 4, 5, 6]
147	PFIETSAKTRQRVED	ubiquitination	[4, 7]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

NP_BIND 10 17 GTP.
NP_BIND 57 61 GTP.
NP_BIND 116 119 GTP.
REGION 166 185 Hypervariable region.
MOTIF 32 40 Effector region.
MOD_RES 1 1 N-acetylmethionine; in GTPase KRas;
MOD_RES 2 2 N-acetylthreonine; in GTPase KRas, N-
MOD_RES 104 104 N6-acetyllysine.
MOD_RES 186 186 Cysteine methyl ester.
LIPID 180 180 S-palmitoyl cysteine.
LIPID 186 186 S-farnesyl cysteine.

3D-structure; Acetylation; Alternative splicing; Cardiomyopathy; Cell membrane; Complete proteome; Deafness; Direct protein sequencing; Disease mutation; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Palmitate; Polymorphism; Prenylation; Proto-oncogene; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0045121; C:membrane raft; IEA:Compara.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0019003; F:GDP binding; IEA:Compara.
GO:0019002; F:GMP binding; IEA:Compara.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; IEA:InterPro.
GO:0000186; P:activation of MAPKK activity; TAS:Reactome.
GO:0007411; P:axon guidance; TAS:Reactome.
GO:0007596; P:blood coagulation; TAS:Reactome.
GO:0019221; P:cytokine-mediated signaling pathway; IEA:Compara.
GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
GO:0050900; P:leukocyte migration; TAS:Reactome.
GO:0000165; P:MAPK cascade; TAS:Reactome.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0010628; P:positive regulation of gene expression; IMP:BHF-UCL.
GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IEA:Compara.
GO:0051000; P:positive regulation of nitric-oxide synthase activity; IEA:Compara.
GO:0007265; P:Ras protein signal transduction; TAS:Reactome.
GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
GO:0051385; P:response to mineralocorticoid stimulus; IEA:Compara.
GO:0035176; P:social behavior; IEA:Compara.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR020849; Small_GTPase_Ras.

PR00449; RASTRNSFRMNG.