CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000386
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-related protein 2/3 complex subunit 2 
Protein Synonyms/Alias
 Arp2/3 complex 34 kDa subunit; p34-ARC 
Gene Name
 ARPC2 
Gene Synonyms/Alias
 ARC34; PRO2446 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65SISLKFYKELQAHGAubiquitination[1, 2, 3, 4]
77HGADELLKRVYGSFLubiquitination[3]
117VHQAGMLKRNCFASVubiquitination[5, 6]
127CFASVFEKYFQFQEEubiquitination[3]
136FQFQEEGKEGENRAVubiquitination[3, 5, 7]
157ETMYVESKKDRVTVVubiquitination[5]
186KVFMQEFKEGRRASHacetylation[7]
186KVFMQEFKEGRRASHubiquitination[3, 5]
256DYLHYHIKCSKAYIHubiquitination[3]
259HYHIKCSKAYIHTRMubiquitination[3]
269IHTRMRAKTSDFLKVubiquitination[3]
275AKTSDFLKVLNRARPacetylation[8]
275AKTSDFLKVLNRARPubiquitination[1, 2, 3, 4, 5, 9]
290DAEKKEMKTITGKTFubiquitination[3]
295EMKTITGKTFSSR**acetylation[8]
295EMKTITGKTFSSR**ubiquitination[1, 2, 3, 4, 7, 9]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. 
Sequence Annotation
 MOD_RES 275 275 N6-acetyllysine.
 MOD_RES 295 295 N6-acetyllysine.  
Keyword
 Acetylation; Actin-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 300 AA 
Protein Sequence
MILLEVNNRI IEETLALKFE NAAAGNKPEA VEVTFADFDG VLYHISNPNG DKTKVMVSIS 60
LKFYKELQAH GADELLKRVY GSFLVNPESG YNVSLLYDLE NLPASKDSIV HQAGMLKRNC 120
FASVFEKYFQ FQEEGKEGEN RAVIHYRDDE TMYVESKKDR VTVVFSTVFK DDDDVVIGKV 180
FMQEFKEGRR ASHTAPQVLF SHREPPLELK DTDAAVGDNI GYITFVLFPR HTNASARDNT 240
INLIHTFRDY LHYHIKCSKA YIHTRMRAKT SDFLKVLNRA RPDAEKKEMK TITGKTFSSR 300 
Gene Ontology
 GO:0005885; C:Arp2/3 protein complex; TAS:ProtInc.
 GO:0031252; C:cell leading edge; IEA:Compara.
 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IEA:Compara. 
Interpro
 IPR007188; P34-arc. 
Pfam
 PF04045; P34-Arc 
SMART
  
PROSITE
  
PRINTS