CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010482
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyruvate kinase 
Protein Synonyms/Alias
 PK; Vegetative protein 17; VEG17 
Gene Name
 pyk 
Gene Synonyms/Alias
 pykA; BSU29180 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
173NLPGITEKDARDIVFacetylation[1]
272KKCNALGKPVITATQacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
  
Sequence Annotation
 METAL 34 34 Potassium (By similarity).
 METAL 36 36 Potassium (By similarity).
 METAL 66 66 Potassium (By similarity).
 METAL 67 67 Potassium; via carbonyl oxygen (By
 METAL 222 222 Magnesium (By similarity).
 METAL 246 246 Magnesium (By similarity).
 BINDING 32 32 Substrate (By similarity).
 BINDING 245 245 Substrate; via amide nitrogen (By
 BINDING 246 246 Substrate; via amide nitrogen (By
 BINDING 278 278 Substrate (By similarity).  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Potassium; Pyruvate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MRKTKIVCTI GPASESIEML TKLMESGMNV ARLNFSHGDF EEHGARIKNI REASKKLGKN 60
VGILLDTKGP EIRTHTMENG GIELETGKEL IISMDEVVGT TDKISVTYEG LVHDVEQGST 120
ILLDDGLIGL EVLDVDAAKR EIKTKVLNNG TLKNKKGVNV PGVSVNLPGI TEKDARDIVF 180
GIEQGVDFIA PSFIRRSTDV LEIRELLEEH NAQDIQIIPK IENQEGVDNI DAILEVSDGL 240
MVARGDLGVE IPAEEVPLVQ KELIKKCNAL GKPVITATQM LDSMQRNPRP TRAEASDVAN 300
AIFDGTDAIM LSGETAAGSY PVEAVQTMHN IASRSEEALN YKEILSKRRD QVGMTITDAI 360
GQSVAHTAIN LNAAAIVTPT ESGHTARMIA KYRPQAPIVA VTVNDSISRK LALVSGVFAE 420
SGQNASSTDE MLEDAVQKSL NSGIVKHGDL IVITAGTVGE SGTTNLMKVH TVGDIIAKGQ 480
GIGRKSAYGP VVVAQNAKEA EQKMTDGAVL VTKSTDRDMI ASLEKASALI TEEGGLTSHA 540
AVVGLSLGIP VIVGLENATS ILTDGQDITV DASRGAVYQG RASVL 585 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR008279; PEP-util_enz_mobile_dom.
 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015795; Pyrv_Knase_C.
 IPR015806; Pyrv_Knase_insert_dom. 
Pfam
 PF00391; PEP-utilizers
 PF00224; PK
 PF02887; PK_C 
SMART
  
PROSITE
 PS00110; PYRUVATE_KINASE 
PRINTS
 PR01050; PYRUVTKNASE.