CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005090
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-galactosidase 
Protein Synonyms/Alias
 Lactase 
Gene Name
 lacS 
Gene Synonyms/Alias
 SSO3019 
Created Date
 July 27, 2013 
Organism
 Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322  
NCBI Taxa ID
 273057 
Lysine Modification
Position
Peptide
Type
References
116EINENELKRLDEYANmethylation[1, 2]
135NHYREIFKDLKSRGLmethylation[1, 2]
273SFQPLTDKDMEAVEMmethylation[1, 2]
311KIVRDDLKGRLDWIGmethylation[1, 2]
332TVVKRTEKGYVSLGGmethylation[1, 2]
Reference
 [1] Thermal stability and aggregation of sulfolobus solfataricus beta-glycosidase are dependent upon the N-epsilon-methylation of specific lysyl residues: critical role of in vivo post-translational modifications.
 Febbraio F, Andolfo A, Tanfani F, Briante R, Gentile F, Formisano S, Vaccaro C, Scirè A, Bertoli E, Pucci P, Nucci R.
 J Biol Chem. 2004 Mar 12;279(11):10185-94. [PMID: 14660666]
 [2] Update on activities at the Universal Protein Resource (UniProt) in 2013.
 e="String">UniProt Consortium.
 Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681] 
Functional Description
  
Sequence Annotation
 ACT_SITE 206 206 Proton donor (Potential).
 ACT_SITE 387 387 Nucleophile (By similarity).
 MOD_RES 116 116 N6-methyllysine; partial.
 MOD_RES 135 135 N6-methyllysine.
 MOD_RES 273 273 N6-methyllysine; partial.
 MOD_RES 311 311 N6-methyllysine; partial.
 MOD_RES 332 332 N6-methyllysine.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Glycosidase; Hydrolase; Methylation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 489 AA 
Protein Sequence
MYSFPNSFRF GWSQAGFQSE MGTPGSEDPN TDWYKWVHDP ENMAAGLVSG DLPENGPGYW 60
GNYKTFHDNA QKMGLKIARL NVEWSRIFPN PLPRPQNFDE SKQDVTEVEI NENELKRLDE 120
YANKDALNHY REIFKDLKSR GLYFILNMYH WPLPLWLHDP IRVRRGDFTG PSGWLSTRTV 180
YEFARFSAYI AWKFDDLVDE YSTMNEPNVV GGLGYVGVKS GFPPGYLSFE LSRRAMYNII 240
QAHARAYDGI KSVSKKPVGI IYANSSFQPL TDKDMEAVEM AENDNRWWFF DAIIRGEITR 300
GNEKIVRDDL KGRLDWIGVN YYTRTVVKRT EKGYVSLGGY GHGCERNSVS LAGLPTSDFG 360
WEFFPEGLYD VLTKYWNRYH LYMYVTENGI ADDADYQRPY YLVSHVYQVH RAINSGADVR 420
GYLHWSLADN YEWASGFSMR FGLLKVDYNT KRLYWRPSAL VYREIATNGA ITDEIEHLNS 480
VPPVKPLRH 489 
Gene Ontology
 GO:0004565; F:beta-galactosidase activity; IEA:EC.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. 
Interpro
 IPR001360; Glyco_hydro_1.
 IPR018120; Glyco_hydro_1_AS.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00232; Glyco_hydro_1 
SMART
  
PROSITE
 PS00572; GLYCOSYL_HYDROL_F1_1
 PS00653; GLYCOSYL_HYDROL_F1_2 
PRINTS
 PR00131; GLHYDRLASE1.