UniProt Accession

 T1S_SALTY; P06187 

Genbank Protein ID

 Y00524; AE006468; K03551 

Genbank Nucleotide ID

 CAA68580.1; AAL23342.1; AAA27146.1 

Protein Name

 Type-1 restriction enzyme StySJI specificity protein 

Protein Synonyms/Alias

 S.StySJI; Type I restriction enzyme StySJI specificity protein; S protein 

Gene Name

 hsdS 

Gene Synonyms/Alias

 STM4524 

Created Date

 July 27, 2013 

Organism

 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 

NCBI Taxa ID

 99287 

Lysine Modification

Position	Peptide	Type	References
247	KGGKRLPKGESLIAE	acetylation	[1]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The StySJI enzyme recognizes 5'- GAGN(6)GTRC-3'. 

Sequence Annotation

  

Keyword

 Complete proteome; DNA-binding; Reference proteome; Restriction system. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 469 AA 

Protein Sequence

Gene Ontology

 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW. 

Interpro

 IPR000055; Restrct_endonuc_typeI_HsdS. 

Pfam

 PF01420; Methylase_S 

SMART

  

PROSITE

  

PRINTS