CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018331
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable tRNA pseudouridine synthase 1 
Protein Synonyms/Alias
  
Gene Name
 TRUB1 
Gene Synonyms/Alias
 PUS4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
147TSMLSGSKRYTAIGEubiquitination[1]
157TAIGELGKATDTLDSubiquitination[1]
172TGRVTEEKPYDKITQubiquitination[1]
176TEEKPYDKITQEDIEubiquitination[1]
205PLYSALKKDGQRLSTubiquitination[1]
264SLVSDIGKELSSCANubiquitination[1]
321ELALKKSKPESNEQVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May be responsible for synthesis of pseudouridine from uracil in transfer RNAs (By similarity). 
Sequence Annotation
 ACT_SITE 121 121 Nucleophile (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 11 11 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Isomerase; Phosphoprotein; Polymorphism; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 349 AA 
Protein Sequence
MAASEAAVVS SPSLKTDTSP VLETAGTVAA MAATPSARAA AAVVAAAART GSEARVSKAA 60
LATKLLSLSG VFAVHKPKGP TSAELLNRLK EKLLAEAGMP SPEWTKRKKQ TLKIGHGGTL 120
DSAARGVLVV GIGSGTKMLT SMLSGSKRYT AIGELGKATD TLDSTGRVTE EKPYDKITQE 180
DIEGILQKFT GNIMQVPPLY SALKKDGQRL STLMKRGEVV EAKPARPVTV YSISLQKFQP 240
PFFTLDVECG GGFYIRSLVS DIGKELSSCA NVLELTRTKQ GPFTLEEHAL PEDKWTIDDI 300
AQSLEHCSSL FPAELALKKS KPESNEQVLS CEYITLNEPK REDDVIKTC 349 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom.
 IPR014780; tRNA_psdUridine_synth_TruB. 
Pfam
 PF01509; TruB_N 
SMART
  
PROSITE
  
PRINTS