CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aminopeptidase B 
Protein Synonyms/Alias
 AP-B; Arginine aminopeptidase; Arginyl aminopeptidase 
Gene Name
 RNPEP 
Gene Synonyms/Alias
 APB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
161APEQTAGKKKPFVYTubiquitination[1]
162PEQTAGKKKPFVYTQubiquitination[1, 2]
163EQTAGKKKPFVYTQGubiquitination[1]
394GEENPLNKLRVKIEPubiquitination[3]
398PLNKLRVKIEPGVDPubiquitination[1, 2, 3]
446KAYVHEFKFRSILADacetylation[4]
446KAYVHEFKFRSILADubiquitination[1]
600EFLHNQGKQKYTLPLubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(- 1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity). 
Sequence Annotation
 REGION 298 302 Substrate binding (By similarity).
 ACT_SITE 326 326 Proton acceptor (By similarity).
 METAL 325 325 Zinc; catalytic (By similarity).
 METAL 329 329 Zinc; catalytic (By similarity).
 METAL 348 348 Zinc; catalytic (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 446 446 N6-acetyllysine.  
Keyword
 Acetylation; Aminopeptidase; Complete proteome; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Polymorphism; Protease; Reference proteome; Secreted; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 650 AA 
Protein Sequence
MASGEHSPGS GAARRPLHSA QAVDVASASN FRAFELLHLH LDLRAEFGPP GPGAGSRGLS 60
GTAVLDLRCL EPEGAAELRL DSHPCLEVTA AALRRERPGS EEPPAEPVSF YTQPFSHYGQ 120
ALCVSFPQPC RAAERLQVLL TYRVGEGPGV CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP 180
CFDTPAVKYK YSALIEVPDG FTAVMSASTW EKRGPNKFFF QMCQPIPSYL IALAIGDLVS 240
AEVGPRSRVW AEPCLIDAAK EEYNGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM 300
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF TMYAQRRIST 360
ILFGAAYTCL EAATGRALLR QHMDITGEEN PLNKLRVKIE PGVDPDDTYN ETPYEKGFCF 420
VSYLAHLVGD QDQFDSFLKA YVHEFKFRSI LADDFLDFYL EYFPELKKKR VDIIPGFEFD 480
RWLNTPGWPP YLPDLSPGDS LMKPAEELAQ LWAAEELDMK AIEAVAISPW KTYQLVYFLD 540
KILQKSPLPP GNVKKLGDTY PSISNARNAE LRLRWGQIVL KNDHQEDFWK VKEFLHNQGK 600
QKYTLPLYHA MMGGSEVAQT LAKETFASTA SQLHSNVVNY VQQIVAPKGS 650 
Gene Ontology
 GO:0005576; C:extracellular region; ISS:UniProtKB.
 GO:0005615; C:extracellular space; IEA:InterPro.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0004177; F:aminopeptidase activity; ISS:UniProtKB.
 GO:0004301; F:epoxide hydrolase activity; NAS:UniProtKB.
 GO:0008235; F:metalloexopeptidase activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0019370; P:leukotriene biosynthetic process; IEA:InterPro.
 GO:0006508; P:proteolysis; IC:UniProtKB. 
Interpro
 IPR016024; ARM-type_fold.
 IPR015571; Pept_M1_aminopeptidase-B.
 IPR001930; Peptidase_M1.
 IPR015211; Peptidase_M1_C.
 IPR014782; Peptidase_M1_N. 
Pfam
 PF09127; Leuk-A4-hydro_C
 PF01433; Peptidase_M1 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00756; ALADIPTASE.