UniProt Accession

 ADHX_RAT; P12711; Q5XIF8 

Genbank Protein ID

 BC083724 

Genbank Nucleotide ID

 AAH83724.1 

Protein Name

 Alcohol dehydrogenase class-3 

Protein Synonyms/Alias

 Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; ADH-B2; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase 

Gene Name

 Adh5 

Gene Synonyms/Alias

 Adh-2; Adh2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
226	IIGIDINKDKFAKAK	acetylation	[1]
228	GIDINKDKFAKAKEF	acetylation	[1]
330	KSVESVPKLVSEYMS	acetylation	[1]
338	LVSEYMSKKIKVDEF	acetylation	[1]
357	LSFDQINKAFDLMHS	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. 

Sequence Annotation

 METAL 45 45 Zinc 1; catalytic (By similarity).
 METAL 67 67 Zinc 1; catalytic (By similarity).
 METAL 97 97 Zinc 2 (By similarity).
 METAL 100 100 Zinc 2 (By similarity).
 METAL 103 103 Zinc 2 (By similarity).
 METAL 111 111 Zinc 2 (By similarity).
 METAL 174 174 Zinc 1; catalytic (By similarity).
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 374 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:RGD.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0004022; F:alcohol dehydrogenase (NAD) activity; IDA:RGD.
 GO:0005504; F:fatty acid binding; IEA:Compara.
 GO:0018467; F:formaldehyde dehydrogenase activity; IEA:Compara.
 GO:0051903; F:S-(hydroxymethyl)glutathione dehydrogenase activity; IEA:EC.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007568; P:aging; IEP:RGD.
 GO:0006068; P:ethanol catabolic process; IDA:RGD.
 GO:0006069; P:ethanol oxidation; IEA:InterPro.
 GO:0046294; P:formaldehyde catabolic process; IEA:Compara.
 GO:0018119; P:peptidyl-cysteine S-nitrosylation; IEA:Compara.
 GO:0045777; P:positive regulation of blood pressure; IEA:Compara.
 GO:0003016; P:respiratory system process; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0051409; P:response to nitrosative stress; IEA:Compara.
 GO:0051775; P:response to redox state; IEA:Compara.
 GO:0001523; P:retinoid metabolic process; IEA:Compara. 

Interpro

 IPR014183; ADH_3.
 IPR013149; ADH_C.
 IPR013154; ADH_GroES-like.
 IPR002085; ADH_SF_Zn-type.
 IPR002328; ADH_Zn_CS.
 IPR011032; GroES-like.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF08240; ADH_N
 PF00107; ADH_zinc_N 

SMART

  

PROSITE

 PS00059; ADH_ZINC 

PRINTS