UniProt Accession

 INT7_HUMAN; Q9NVH2; B4DLZ6; B7WNP6; B7WPB6; Q8N4K7; Q8WUH5; Q9H9V3; Q9NVU5; Q9UFC6; Q9UFM3 

Genbank Protein ID

 AK001363; AK001598; AK022509; AK022589; AK297225; AL117576; AM392654; AC092814; CH471100; BC020523; BC030716; BC033918; AL133049; BK005725 

Genbank Nucleotide ID

 BAA91650.1; BAA91779.1; BAB14067.1; BAB14116.1; BAG59708.1; CAB56000.1; CAL37532.1; EAW93406.1; AAH20523.1; AAH30716.1; AAH33918.1; CAB61376.1; DAA05725.1 

Protein Name

 Integrator complex subunit 7 

Protein Synonyms/Alias

 Int7 

Gene Name

 INTS7 

Gene Synonyms/Alias

 C1orf73 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
31	SALMELDKGLRSGKL	ubiquitination	[1]
37	DKGLRSGKLGEQCEA	ubiquitination	[2, 3]
105	LNVDEFVKRIFSVIH	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
194	LATPVDLKLKLIPIL	ubiquitination	[1]
277	RLAIQDLKLLANKTP	ubiquitination	[1, 2, 3, 5, 8]
282	DLKLLANKTPHTWSR	ubiquitination	[2]
346	PRSSDLVKLAQECCY	ubiquitination	[2, 5]
510	KALSVESKAVIKQQL	ubiquitination	[2]
514	VESKAVIKQQLESVS	ubiquitination	[2, 5]
672	GRISNQMKQSMEEFR	ubiquitination	[2, 3, 8]
798	FQRYFFQKLQSTSIK	ubiquitination	[5]
805	KLQSTSIKLALSPSP	ubiquitination	[1]
840	GVVQHGSKPGLFRKI	ubiquitination	[3, 4, 9]
867	SKSGQDYKIPIDNMT	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11]
929	PRTTIFVKSLEDPYS	ubiquitination	[1, 3, 8, 11]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [11] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase. 

Sequence Annotation

 MOD_RES 525 525 Phosphothreonine (By similarity).
 MOD_RES 527 527 Phosphotyrosine (By similarity).  

Keyword

 Alternative splicing; Chromosome; Complete proteome; DNA damage; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 962 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IDA:UniProtKB.
 GO:0032039; C:integrator complex; IDA:HGNC.
 GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
 GO:0000077; P:DNA damage checkpoint; IMP:UniProtKB.
 GO:0016180; P:snRNA processing; IDA:HGNC. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold. 

Pfam

  

SMART

  

PROSITE

  

PRINTS