UniProt Accession

 AK1D1_HUMAN; P51857; A1L4P6; A8K060; B4DPN3; B4DPN8 

Genbank Protein ID

 Z28339; AF283659; AF283651; AF283652; AF283653; AF283654; AF283655; AF283656; AF283657; AF283658; AK289425; AK298421; AK298428; AC009263; AC024082; AC083867; CH236950; CH471070; BC130625; BC130627 

Genbank Nucleotide ID

 CAA82193.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; AAG39381.1; BAF82114.1; BAG60645.1; BAG60650.1; EAL24049.1; EAW83881.1; AAI30626.1; AAI30628.1 

Protein Name

 3-oxo-5-beta-steroid 4-dehydrogenase 

Protein Synonyms/Alias

 Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase 

Gene Name

 AKR1D1 

Gene Synonyms/Alias

 SRD5B1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
71	VGEAIREKIAEGKVR	acetylation	[1]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. 

Sequence Annotation

 NP_BIND 169 170 NADP.
 NP_BIND 220 224 NADP.
 NP_BIND 273 283 NADP.
 ACT_SITE 58 58 Proton donor.
 BINDING 53 53 NADP.
 BINDING 193 193 NADP.
 BINDING 230 230 Substrate.  

Keyword

 3D-structure; Alternative splicing; Bile acid catabolism; Complete proteome; Cytoplasm; Disease mutation; Intrahepatic cholestasis; Lipid degradation; Lipid metabolism; NADP; Oxidoreductase; Reference proteome; Steroid metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 326 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0047787; F:delta4-3-oxosteroid 5beta-reductase activity; IEA:EC.
 GO:0005496; F:steroid binding; TAS:UniProtKB.
 GO:0008209; P:androgen metabolic process; IDA:UniProtKB.
 GO:0006699; P:bile acid biosynthetic process; IDA:UniProtKB.
 GO:0030573; P:bile acid catabolic process; IEA:UniProtKB-KW.
 GO:0008207; P:C21-steroid hormone metabolic process; IDA:UniProtKB.
 GO:0006707; P:cholesterol catabolic process; IDA:UniProtKB.
 GO:0007586; P:digestion; IDA:UniProtKB.
 GO:0055114; P:oxidation-reduction process; IDA:UniProtKB. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.