UniProt Accession

 SUMF1_HUMAN; Q8NBK3; B4DXK5; B7XD05; E9PGL0; G5E9B0; Q0VAC6; Q0VAC7; Q2NL78; Q53ZE4; Q6UY39; Q96AK5; Q96DK8 

Genbank Protein ID

 AY208752; AY323910; AB448737; AY358092; AK057983; AK302018; AK075459; AC018822; AC023480; AC023483; AC023484; AC024167; AC024168; AC034191; CH471055; CH471055; BC017005; BC110862; BC121122; BC121123 

Genbank Nucleotide ID

 AAO34683.1; AAP86217.1; BAH11168.1; AAQ88459.1; BAB71625.1; BAG63417.1; BAC11634.1; EAW63906.1; EAW63907.1; AAH17005.2; AAI10863.1; AAI21123.1; AAI21124.1 

Protein Name

 Sulfatase-modifying factor 1 

Protein Synonyms/Alias

 C-alpha-formylglycine-generating enzyme 1 

Gene Name

 SUMF1 

Gene Synonyms/Alias

 FGE; PSEC0152; UNQ3037/PRO9852 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
249	RLFPWGNKLQPKGQH	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. 

Sequence Annotation

 REGION 341 360 Interaction with sulfatases.
 ACT_SITE 333 333 Proton acceptor (Probable).
 METAL 130 130 Calcium 2.
 METAL 259 259 Calcium 1.
 METAL 260 260 Calcium 1; via carbonyl oxygen.
 METAL 273 273 Calcium 1.
 METAL 275 275 Calcium 1; via carbonyl oxygen.
 METAL 293 293 Calcium 2; via carbonyl oxygen.
 METAL 296 296 Calcium 2; via carbonyl oxygen.
 METAL 298 298 Calcium 2; via carbonyl oxygen.
 METAL 300 300 Calcium 2.
 CARBOHYD 141 141 N-linked (GlcNAc...).
 DISULFID 50 52
 DISULFID 218 365
 DISULFID 235 346
 DISULFID 336 341 Redox-active.  

Keyword

 3D-structure; Alternative splicing; Calcium; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Ichthyosis; Leukodystrophy; Metachromatic leukodystrophy; Metal-binding; Mucopolysaccharidosis; Oxidoreductase; Polymorphism; Redox-active center; Reference proteome; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 374 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR016187; C-type_lectin_fold.
 IPR005532; FGE_dom. 

Pfam

 PF03781; FGE-sulfatase 

SMART

  

PROSITE

  

PRINTS