CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010965
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-6 
Protein Synonyms/Alias
 Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform; MyHC-alpha 
Gene Name
 Myh6 
Gene Synonyms/Alias
 Myhca 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
206AAIGDRSKKENPNANubiquitination[1]
207AIGDRSKKENPNANKubiquitination[1]
214KENPNANKGTLEDQIubiquitination[1]
1167SVQIEMNKKREAEFQubiquitination[2]
1227EKEKSEFKLELDDVTubiquitination[2]
1242SNMEQIIKAKANLEKubiquitination[2]
1249KAKANLEKVSRTLEDubiquitination[2]
1264QANEYRVKLEEAQRSubiquitination[2]
1281DFTTQRAKLQTENGEubiquitination[1, 2]
1296LARQLEEKEALISQLubiquitination[2]
1307ISQLTRGKLSYTQQMubiquitination[2]
1318TQQMEDLKRQLEEEGubiquitination[2]
1326RQLEEEGKAKNALAHubiquitination[2]
1356YEEEMEAKAELQRVLubiquitination[2]
1365ELQRVLSKANSEVAQubiquitination[2]
1412AVEAVNAKCSSLEKTubiquitination[2]
1418AKCSSLEKTKHRLQNubiquitination[2]
1446AAAAALDKKQRNFDKubiquitination[2]
1447AAAALDKKQRNFDKIubiquitination[2]
1459DKILAEWKQKYEESQubiquitination[2]
1474SELESSQKEARSLSTubiquitination[2]
1487STELFKLKNAYEESLubiquitination[2]
1501LEHLETFKRENKNLQubiquitination[2]
1505ETFKRENKNLQEEISubiquitination[2]
1523EQLGEGGKNVHELEKubiquitination[2]
1530KNVHELEKIRKQLEVubiquitination[2]
1533HELEKIRKQLEVEKLubiquitination[2]
1539RKQLEVEKLELQSALubiquitination[1]
1559SLEHEEGKILRAQLEubiquitination[2]
1571QLEFNQIKAEIERKLubiquitination[1, 2]
1577IKAEIERKLAEKDEEubiquitination[2]
1581IERKLAEKDEEMEQAubiquitination[2]
1589DEEMEQAKRNHLRMVubiquitination[2]
1618NEALRVKKKMEGDLNubiquitination[2]
1643RIASEAQKHLKNSQAubiquitination[2]
1646SEAQKHLKNSQAHLKubiquitination[2]
1653KNSQAHLKDTQLQLDubiquitination[2]
1670VHANDDLKENIAIVEubiquitination[2]
1702EQTERSRKLAEQELIubiquitination[2]
1729NTSLINQKKKMESDLubiquitination[2]
1786AHLERMKKNMEQTIKubiquitination[2]
1793KNMEQTIKDLQHRLDubiquitination[2]
1812IALKGGKKQLQKLEAubiquitination[2]
1816GGKKQLQKLEARVREubiquitination[2]
1833NELEAEQKRNAESVKubiquitination[2]
1840KRNAESVKGMRKSERubiquitination[2]
1850RKSERRIKELTYQTEubiquitination[2]
1860TYQTEEDKKNLMRLQubiquitination[2]
1861YQTEEDKKNLMRLQDubiquitination[2]
1872RLQDLVDKLQLKVKAubiquitination[2]
1876LVDKLQLKVKAYKRQubiquitination[2]
1878DKLQLKVKAYKRQAEubiquitination[2]
1897QANTNLSKFRKVQHEubiquitination[2]
1900TNLSKFRKVQHELDEubiquitination[2]
1921IAESQVNKLRAKSRDubiquitination[2]
1932KSRDIGAKKMHDEE*ubiquitination[2]
1933SRDIGAKKMHDEE**ubiquitination[2]
Reference
 [1] A proteomics approach to identify the ubiquitinated proteins in mouse heart.
 Jeon HB, Choi ES, Yoon JH, Hwang JH, Chang JW, Lee EK, Choi HW, Park ZY, Yoo YJ.
 Biochem Biophys Res Commun. 2007 Jun 8;357(3):731-6. [PMID: 17451654]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Muscle contraction. 
Sequence Annotation
 DOMAIN 1 782 Myosin head-like.
 DOMAIN 783 812 IQ.
 NP_BIND 178 185 ATP.
 REGION 657 679 Actin-binding.
 REGION 759 773 Actin-binding.
 REGION 790 807 Calmodulin-binding (By similarity).
 REGION 816 833 Calmodulin-binding (By similarity).
 MOD_RES 129 129 N6,N6,N6-trimethyllysine (Potential).
 CROSSLNK 206 206 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 207 207 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 214 214 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1281 1281 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1539 1539 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1571 1571 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Polymorphism; Reference proteome; Thick filament; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1938 AA 
Protein Sequence
MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEYVKAK VVSREGGKVT 60
AETENGKTVT IKEDQVMQQN PPKFDKIEDM AMLTFLHEPA VLYNLKERYA AWMIYTYSGL 120
FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES 180
GAGKTVNTKR VIQYFASIAA IGDRSKKENP NANKGTLEDQ IIQANPALEA FGNAKTVRND 240
NSSRFGKFIR IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD 300
MLLVTNNPYD YAFVSQGEVS VASIDDSEEL LATDSAFDVL SFTAEEKAGV YKLTGAIMHY 360
GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL CHPRVKVGNE YVTKGQSVQQ 420
VYYSIGALAK SVYEKMFNWM VTRINATLET KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN 480
FTNEKLQQFF NHHMFVLEQE EYKKEGIEWE FIDFGMDLQA CIDLIEKPMG IMSILEEECM 540
FPKASDMTFK AKLYDNHLGK SNNFQKPRNV KGKQEAHFSL VHYAGTVDYN IMGWLEKNKD 600
PLNETVVGLY QKSSLKLMAT LFSTYASADT GDSGKGKGGK KKGSSFQTVS ALHRENLNKL 660
MTNLKTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV LEGIRICRKG FPNRILYGDF 720
RQRYRILNPA AIPEGQFIDS RKGAEKLLGS LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD 780
ERLSRIITRI QAQARGQLMR IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP 840
LLKSAETEKE MANMKEEFGR VKDALEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL 900
NDAEERCDQL IKNKIQLEAK VKEMTERLED EEEMNAELTA KKRKLEDECS ELKKDIDDLE 960
LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA LQEAHQQALD DLQAEEDKVN 1020
TLTKSKVKLE QQVDDLEGSL EQEKKVRMDL ERAKRKLEGD LKLTQESIMD LENDKLQLEE 1080
KLKKKEFDIS QQNSKIEDEQ ALALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD 1140
LSRELEEISE RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD 1200
SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV SRTLEDQANE 1260
YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI SQLTRGKLSY TQQMEDLKRQ 1320
LEEEGKAKNA LAHALQSSRH DCDLLREQYE EEMEAKAELQ RVLSKANSEV AQWRTKYETD 1380
AIQRTEELEE AKKKLAQRLQ DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA 1440
AAALDKKQRN FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF 1500
KRENKNLQEE ISDLTEQLGE GGKNVHELEK IRKQLEVEKL ELQSALEEAE ASLEHEEGKI 1560
LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHLRMVDSLQ TSLDAETRSR NEALRVKKKM 1620
EGDLNEMEIQ LSQANRIASE AQKHLKNSQA HLKDTQLQLD DAVHANDDLK ENIAIVERRN 1680
NLLQAELEEL RAVVEQTERS RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ 1740
TEVEEAVQEC RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD 1800
EAEQIALKGG KKQLQKLEAR VRELENELEA EQKRNAESVK GMRKSERRIK ELTYQTEEDK 1860
KNLMRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK VQHELDEAEE RADIAESQVN 1920
KLRAKSRDIG AKKMHDEE 1938 
Gene Ontology
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0016459; C:myosin complex; IDA:MGI.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0001725; C:stress fiber; IDA:MGI.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0030898; F:actin-dependent ATPase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000146; F:microfilament motor activity; IMP:MGI.
 GO:0008307; F:structural constituent of muscle; IEA:Compara.
 GO:0030048; P:actin filament-based movement; IMP:MGI.
 GO:0007512; P:adult heart development; IMP:MGI.
 GO:0055009; P:atrial cardiac muscle tissue morphogenesis; IEA:Compara.
 GO:0030509; P:BMP signaling pathway; IDA:MGI.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IDA:MGI.
 GO:0048739; P:cardiac muscle fiber development; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0030049; P:muscle filament sliding; IEA:Compara.
 GO:0043462; P:regulation of ATPase activity; IMP:MGI.
 GO:0008217; P:regulation of blood pressure; IMP:MGI.
 GO:0060420; P:regulation of heart growth; IDA:MGI.
 GO:0002027; P:regulation of heart rate; IMP:MGI.
 GO:0002026; P:regulation of the force of heart contraction; IMP:MGI.
 GO:0045214; P:sarcomere organization; IMP:MGI.
 GO:0006941; P:striated muscle contraction; IMP:MGI.
 GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IEA:Compara.
 GO:0007522; P:visceral muscle development; IMP:MGI. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.