UniProt Accession

 DGKE_HUMAN; P52429; Q9UKQ3 

Genbank Protein ID

 U49379; AF136745 

Genbank Nucleotide ID

 AAC50497.1; AAD45666.1 

Protein Name

 Diacylglycerol kinase epsilon 

Protein Synonyms/Alias

 DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon 

Gene Name

 DGKE 

Gene Synonyms/Alias

 DAGK5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
109	ADKRFQCKEIMLKND	ubiquitination	[1]
215	VLASKLGKQWTPLII	ubiquitination	[1]
254	VQVFDVTKTPPIKAL	ubiquitination	[1]
357	WKVQVTNKGYYNLRK	ubiquitination	[1, 2, 3]
392	NFHAHREKAPSLFSS	ubiquitination	[1]
404	FSSRILNKAVYLFYG	ubiquitination	[1]
413	VYLFYGTKDCLVQEC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. 

Sequence Annotation

 DOMAIN 215 356 DAGKc.
 ZN_FING 59 108 Phorbol-ester/DAG-type 1.
 ZN_FING 124 177 Phorbol-ester/DAG-type 2.  

Keyword

 ATP-binding; Complete proteome; Kinase; Membrane; Metal-binding; Nucleotide-binding; Polymorphism; Reference proteome; Repeat; Transferase; Transmembrane; Transmembrane helix; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 567 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0004143; F:diacylglycerol kinase activity; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0008654; P:phospholipid biosynthetic process; TAS:ProtInc.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 

Interpro

 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd. 

Pfam

 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat 

SMART

 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc 

PROSITE

 PS50146; DAGK
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS