UniProt Accession

 NUSG_ECOLI; P0AFG0; P16921; Q2M8R8 

Genbank Protein ID

 M30610; U00006; U00096; AP009048 

Genbank Nucleotide ID

 AAA24622.1; AAC43080.1; AAC76956.1; BAE77338.1 

Protein Name

 Transcription termination/antitermination protein NusG 

Protein Synonyms/Alias

  

Gene Name

 nusG 

Gene Synonyms/Alias

 b3982; JW3945 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
106	RPAPISDKEVDAIMN	acetylation	[1]
121	RLQQVGDKPRPKTLF	acetylation	[1]
125	VGDKPRPKTLFEPGE	acetylation	[1]
155	VEEVDYEKSRLKVSV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNA polymerase (RNAP), probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination. 

Sequence Annotation

 DOMAIN 130 161 KOW.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Reference proteome; Transcription; Transcription antitermination; Transcription regulation; Transcription termination. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 181 AA 

Protein Sequence

Gene Ontology

 GO:0006353; P:DNA-dependent transcription, termination; IDA:EcoCyc.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
 GO:0031564; P:transcription antitermination; IDA:EcoCyc. 

Interpro

 IPR005824; KOW.
 IPR014722; Rib_L2_dom2.
 IPR001062; Transcrpt_antiterm_NusG.
 IPR015869; Transcrpt_antiterm_NusG_bac_CS.
 IPR006645; Transcrpt_antiterm_NusG_N.
 IPR008991; Translation_prot_SH3-like. 

Pfam

 PF00467; KOW
 PF02357; NusG 

SMART

 SM00739; KOW
 SM00738; NGN 

PROSITE

 PS01014; NUSG 

PRINTS

 PR00338; NUSGTNSCPFCT.