CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000598
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Disintegrin and metalloproteinase domain-containing protein 10 
Protein Synonyms/Alias
 ADAM 10; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156c 
Gene Name
 Adam10 
Gene Synonyms/Alias
 Kuz; Madm 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
447NISQVLEKKRNNCFVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell- surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP) at '687- Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10- processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity). 
Sequence Annotation
 DOMAIN 221 457 Peptidase M12B.
 DOMAIN 458 552 Disintegrin.
 MOTIF 171 178 Cysteine switch (By similarity).
 MOTIF 709 716 SH3-binding (Potential).
 MOTIF 723 729 SH3-binding (Potential).
 ACT_SITE 385 385 By similarity.
 METAL 173 173 Zinc; in inhibited form (By similarity).
 METAL 384 384 Zinc; catalytic.
 METAL 388 388 Zinc; catalytic.
 METAL 394 394 Zinc; catalytic.
 CARBOHYD 268 268 N-linked (GlcNAc...) (Potential).
 CARBOHYD 279 279 N-linked (GlcNAc...).
 CARBOHYD 440 440 N-linked (GlcNAc...) (Potential).
 CARBOHYD 552 552 N-linked (GlcNAc...) (Potential).
 DISULFID 223 314 By similarity.
 DISULFID 345 452 By similarity.
 DISULFID 400 436 By similarity.
 DISULFID 485 516 By similarity.
 DISULFID 504 512 By similarity.
 DISULFID 525 544 By similarity.
 DISULFID 531 563 By similarity.
 DISULFID 556 568 By similarity.
 DISULFID 573 599 By similarity.
 DISULFID 581 608 By similarity.
 DISULFID 583 598 By similarity.
 DISULFID 595 640 By similarity.
 DISULFID 633 646 By similarity.  
Keyword
 Cleavage on pair of basic residues; Complete proteome; Disulfide bond; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Notch signaling pathway; Protease; Reference proteome; SH3-binding; Signal; Transmembrane; Transmembrane helix; Zinc; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 749 AA 
Protein Sequence
MVLPTVLILL LSWAAGLGGQ YGNPLNKYIR HYEGLSYNVD SLHQKHQRAK RAVSHEDQFL 60
LLDFHAHGRQ FNLRMKRDTS LFSDEFKVET SNKVLDYDTS HIYTGHIYGE EGSFSHGSVI 120
DGRFEGFIKT RGGTFYIEPA ERYIKDRILP FHSVIYHEDD INYPHKYGPQ GGCADHSVFE 180
RMRKYQMTGV EEGARAHPEK HAASSGPELL RKKRTTLAER NTCQLYIQTD HLFFKYYGTR 240
EAVIAQISSH VKAIDTIYQT TDFSGIRNIS FMVKRIRINT TSDEKDPTNP FRFPNIGVEK 300
FLELNSEQNH DDYCLAYVFT DRDFDDGVLG LAWVGAPSGS SGGICEKSKL YSDGKKKSLN 360
TGIITVQNYG SHVPPKVSHI TFAHEVGHNF GSPHDSGTEC TPGESKNLGQ KENGNYIMYA 420
RATSGDKLNN NKFSLCSIRN ISQVLEKKRN NCFVESGQPI CGNGMVEQGE ECDCGYSDQC 480
KDDCCFDANQ PEGKKCKLKP GKQCSPSQGP CCTAQCAFKS KSEKCRDDSD CAKEGICNGF 540
TALCPASDPK PNFTDCNRHT QVCINGQCAG SICEKYDLEE CTCASSDGKD DKELCHVCCM 600
KKMAPSTCAS TGSLQWSKQF SGRTITLQPG SPCNDFRGYC DVFMRCRLVD ADGPLARLKK 660
AIFSPQLYEN IAEWIVAHWW AVLLMGIALI MLMAGFIKIC SVHTPSSNPK LPPPKPLPGT 720
LKRRRPPQPI QQPPRQRPRE SYQMGHMRR 749 
Gene Ontology
 GO:0009986; C:cell surface; ISS:UniProtKB.
 GO:0005798; C:Golgi-associated vesicle; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0014069; C:postsynaptic density; IDA:MGI.
 GO:0004222; F:metalloendopeptidase activity; ISS:UniProtKB.
 GO:0019901; F:protein kinase binding; IDA:UniProtKB.
 GO:0042169; F:SH2 domain binding; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051089; P:constitutive protein ectodomain proteolysis; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IMP:UniProtKB.
 GO:0006509; P:membrane protein ectodomain proteolysis; ISS:UniProtKB.
 GO:0042117; P:monocyte activation; IEA:Compara.
 GO:0007162; P:negative regulation of cell adhesion; ISS:UniProtKB.
 GO:0007220; P:Notch receptor processing; NAS:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IMP:UniProtKB.
 GO:0006913; P:nucleocytoplasmic transport; NAS:UniProtKB.
 GO:0051088; P:PMA-inducible membrane protein ectodomain proteolysis; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0010820; P:positive regulation of T cell chemotaxis; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IDA:UniProtKB.
 GO:0034612; P:response to tumor necrosis factor; IEA:Compara. 
Interpro
 IPR027053; ADAM_10.
 IPR001762; Blood-coag_inhib_Disintegrin.
 IPR024079; MetalloPept_cat_dom.
 IPR001590; Peptidase_M12B.
 IPR002870; Peptidase_M12B_N. 
Pfam
 PF00200; Disintegrin
 PF01562; Pep_M12B_propep 
SMART
 SM00050; DISIN 
PROSITE
 PS50215; ADAM_MEPRO
 PS00546; CYSTEINE_SWITCH
 PS00427; DISINTEGRIN_1
 PS50214; DISINTEGRIN_2
 PS00142; ZINC_PROTEASE 
PRINTS