| Tag | Content |
|---|
| CPLM ID | CPLM-007579 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | PFL-like enzyme TdcE |
Protein Synonyms/Alias | Keto-acid formate acetyltransferase; Keto-acid formate-lyase; Ketobutyrate formate-lyase; KFL; Pyruvate formate-lyase; PFL |
Gene Name | tdcE |
Gene Synonyms/Alias | yhaS; b3114; JW5522 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 458 | INGGVDEKLKIQVGP | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the cleavage of 2-ketobutyrate to propionyl- CoA and formate. It can also use pyruvate as substrate. |
Sequence Annotation | DOMAIN 7 629 PFL.
DOMAIN 636 764 Glycine radical.
ACT_SITE 423 423 S-acetylcysteine intermediate (By
ACT_SITE 424 424 Cysteine radical intermediate (By
MOD_RES 739 739 Glycine radical (By similarity). |
Keyword | Acyltransferase; Complete proteome; Cytoplasm; Organic radical; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 764 AA |
Protein Sequence | MKVDIDTSDK LYADAWLGFK GTDWKNEINV RDFIQHNYTP YEGDESFLAE ATPATTELWE 60
KVMEGIRIEN ATHAPVDFDT NIATTITAHD AGYINQPLEK IVGLQTDAPL KRALHPFGGI 120
NMIKSSFHAY GREMDSEFEY LFTDLRKTHN QGVFDVYSPD MLRCRKSGVL TGLPDGYGRG 180
RIIGDYRRVA LYGISYLVRE RELQFADLQS RLEKGEDLEA TIRLREELAE HRHALLQIQE 240
MAAKYGFDIS RPAQNAQEAV QWLYFAYLAA VKSQNGGAMS LGRTASFLDI YIERDFKAGV 300
LNEQQAQELI DHFIMKIRMV RFLRTPEFDS LFSGDPIWAT EVIGGMGLDG RTLVTKNSFR 360
YLHTLHTMGP APEPNLTILW SEELPIAFKK YAAQVSIVTS SLQYENDDLM RTDFNSDDYA 420
IACCVSPMVI GKQMQFFGAR ANLAKTLLYA INGGVDEKLK IQVGPKTAPL MDDVLDYDKV 480
MDSLDHFMDW LAVQYISALN IIHYMHDKYS YEASLMALHD RDVYRTMACG IAGLSVATDS 540
LSAIKYARVK PIRDENGLAV DFEIDGEYPQ YGNNDERVDS IACDLVERFM KKIKALPTYR 600
NAVPTQSILT ITSNVVYGQK TGNTPDGRRA GTPFAPGANP MHGRDRKGAV ASLTSVAKLP 660
FTYAKDGISY TFSIVPAALG KEDPVRKTNL VGLLDGYFHH EADVEGGQHL NVNVMNREML 720
LDAIEHPEKY PNLTIRVSGY AVRFNALTRE QQQDVISRTF TQAL 764 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:UniProtKB. GO:0016020; C:membrane; IDA:EcoliWiki. GO:0043875; F:2-ketobutyrate formate-lyase activity; IGI:EcoliWiki. GO:0008861; F:formate C-acetyltransferase activity; IDA:EcoCyc. GO:0009061; P:anaerobic respiration; IGI:EcoCyc. GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. GO:0070689; P:L-threonine catabolic process to propionate; IC:EcoliWiki. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |