CPLM-005939

Genbank Nucleotide ID

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Protein Synonyms/Alias

Flavoprotein subunit of complex II; Fp

Homo sapiens (Human)

Position	Peptide	Type	References
41	HFTVDGNKRASAKVS	ubiquitination	[1]
92	FNTACVTKLFPTRSH	ubiquitination	[2, 3]
167	FSRTEDGKIYQRAFG	ubiquitination	[1, 4]
179	AFGGQSLKFGKGGQA	acetylation	[5]
179	AFGGQSLKFGKGGQA	ubiquitination	[1, 2, 4, 6, 7]
250	SIHRIRAKNTVVATG	ubiquitination	[1, 6]
335	ERYAPVAKDLASRDV	acetylation	[5]
335	ERYAPVAKDLASRDV	ubiquitination	[1, 2, 4]
498	ESVMNLDKLRFADGS	acetylation	[8]
538	VLQEGCGKISKLYGD	ubiquitination	[4]
541	EGCGKISKLYGDLKH	acetylation	[5]
547	SKLYGDLKHLKTFDR	acetylation	[5]
547	SKLYGDLKHLKTFDR	ubiquitination	[4]
550	YGDLKHLKTFDRGMV	ubiquitination	[4]
608	IDEYDYSKPIQGQQK	acetylation	[5]
608	IDEYDYSKPIQGQQK	ubiquitination	[1]
616	PIQGQQKKPFEEHWR	ubiquitination	[4]
624	PFEEHWRKHTLSYVD	ubiquitination	[1]

[1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[8] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.

NP_BIND 68 73 FAD (By similarity).
NP_BIND 91 106 FAD (By similarity).
NP_BIND 456 457 FAD (By similarity).
ACT_SITE 340 340 Proton acceptor (By similarity).
BINDING 275 275 FAD (By similarity).
BINDING 296 296 Substrate (By similarity).
BINDING 308 308 Substrate (By similarity).
BINDING 407 407 Substrate (By similarity).
BINDING 440 440 FAD (By similarity).
BINDING 451 451 Substrate (By similarity).
MOD_RES 99 99 Tele-8alpha-FAD histidine (By
MOD_RES 179 179 N6-acetyllysine.
MOD_RES 215 215 Phosphotyrosine; by SRC.
MOD_RES 252 252 Phosphothreonine (By similarity).
MOD_RES 335 335 N6-acetyllysine.
MOD_RES 485 485 N6-acetyllysine (By similarity).
MOD_RES 498 498 N6-acetyllysine (By similarity).
MOD_RES 538 538 N6-acetyllysine (By similarity).
MOD_RES 541 541 N6-acetyllysine.
MOD_RES 547 547 N6-acetyllysine (By similarity).
MOD_RES 608 608 N6-acetyllysine.

Acetylation; Cardiomyopathy; Complete proteome; Direct protein sequencing; Disease mutation; Electron transport; FAD; Flavoprotein; Leigh syndrome; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide; Transport; Tricarboxylic acid cycle; Tumor suppressor.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005749; C:mitochondrial respiratory chain complex II; ISS:UniProtKB.
GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
GO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IEA:EC.
GO:0007399; P:nervous system development; IMP:UniProtKB.
GO:0022904; P:respiratory electron transport chain; IDA:UniProtKB.
GO:0006105; P:succinate metabolic process; IDA:UniProtKB.
GO:0006099; P:tricarboxylic acid cycle; TAS:UniProtKB.

IPR003953; FAD_bind_dom.
IPR003952; FRD_SDH_FAD_BS.
IPR015939; Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477; Succ_DH/fumarate_Rdtase_cat.
IPR011281; Succ_DH_flav_su_fwd.
IPR014006; Succ_Dhase_FrdA_Gneg.

PF00890; FAD_binding_2
PF02910; Succ_DH_flav_C

PS00504; FRD_SDH_FAD_BINDING