UniProt Accession

 E7ENR4_HUMAN; E7ENR4 

Genbank Protein ID

 AC016821; AL596223; AL672126 

Genbank Nucleotide ID

  

Protein Name

 Hexokinase-1 

Protein Synonyms/Alias

  

Gene Name

 HK1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
97	FNPTATVKMLPTFVR	ubiquitination	[1]
182	KRKIKDKKLPVGFTF	ubiquitination	[1]
222	VEGADVVKLLNKAIK	ubiquitination	[1]
226	DVVKLLNKAIKKRGD	ubiquitination	[1]
325	RGSLNPGKQLFEKMV	ubiquitination	[2]
347	LVRLILVKMAKEGLL	ubiquitination	[2]
350	LILVKMAKEGLLFEG	ubiquitination	[1]
368	PELLTRGKFNTSDVS	ubiquitination	[1, 2, 3, 4, 5]
379	SDVSAIEKNKEGLHN	ubiquitination	[2]
381	VSAIEKNKEGLHNAK	ubiquitination	[2]
453	GVDGSLYKTHPQYSR	ubiquitination	[3]
523	KDMLLEVKKRMRAEM	ubiquitination	[1]
524	DMLLEVKKRMRAEME	acetylation	[6]
524	DMLLEVKKRMRAEME	ubiquitination	[1]
773	EYSLNAGKQRYEKMI	ubiquitination	[1, 2]
798	NILIDFTKKGFLFRG	acetylation	[6]
798	NILIDFTKKGFLFRG	ubiquitination	[2, 4]
799	ILIDFTKKGFLFRGQ	ubiquitination	[1]
812	GQISETLKTRGIFET	ubiquitination	[1, 2]
820	TRGIFETKFLSQIES	ubiquitination	[2]
915	RIMHQTVKELSPKCN	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 952 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004396; F:hexokinase activity; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 

Pfam

 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 

SMART

  

PROSITE

 PS00378; HEXOKINASES 

PRINTS

 PR00475; HEXOKINASE.