CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022494
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase MARK1 
Protein Synonyms/Alias
 MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c 
Gene Name
 MARK1 
Gene Synonyms/Alias
 KIAA1477; MARK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
783SGTSIAFKNIASKIAubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). 
Sequence Annotation
 DOMAIN 60 311 Protein kinase.
 DOMAIN 325 370 UBA.
 DOMAIN 746 795 KA1.
 NP_BIND 66 74 ATP (By similarity).
 ACT_SITE 182 182 Proton acceptor (By similarity).
 BINDING 89 89 ATP (By similarity).
 MOD_RES 5 5 Phosphothreonine.
 MOD_RES 215 215 Phosphothreonine; by LKB1 and TAOK1.
 MOD_RES 219 219 Phosphoserine; by GSK3-beta.
 MOD_RES 394 394 Phosphoserine (By similarity).
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 588 588 Phosphoserine.
 MOD_RES 613 613 Phosphothreonine; by PKC/PRKCZ (By
 MOD_RES 666 666 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipid-binding; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 795 AA 
Protein Sequence
MSARTPLPTV NERDTENHTS VDGYTEPHIQ PTKSSSRQNI PRCRNSITSA TDEQPHIGNY 60
RLQKTIGKGN FAKVKLARHV LTGREVAVKI IDKTQLNPTS LQKLFREVRI MKILNHPNIV 120
KLFEVIETEK TLYLVMEYAS GGEVFDYLVA HGRMKEKEAR AKFRQIVSAV QYCHQKYIVH 180
RDLKAENLLL DGDMNIKIAD FGFSNEFTVG NKLDTFCGSP PYAAPELFQG KKYDGPEVDV 240
WSLGVILYTL VSGSLPFDGQ NLKELRERVL RGKYRIPFYM STDCENLLKK LLVLNPIKRG 300
SLEQIMKDRW MNVGHEEEEL KPYTEPDPDF NDTKRIDIMV TMGFARDEIN DALINQKYDE 360
VMATYILLGR KPPEFEGGES LSSGNLCQRS RPSSDLNNST LQSPAHLKVQ RSISANQKQR 420
RFSDHAGPSI PPAVSYTKRP QANSVESEQK EEWDKDVARK LGSTTVGSKS EMTASPLVGP 480
ERKKSSTIPS NNVYSGGSMA RRNTYVCERT TDRYVALQNG KDSSLTEMSV SSISSAGSSV 540
ASAVPSARPR HQKSMSTSGH PIKVTLPTIK DGSEAYRPGT TQRVPAASPS AHSISTATPD 600
RTRFPRGSSS RSTFHGEQLR ERRSVAYNGP PASPSHETGA FAHARRGTST GIISKITSKF 660
VRRDPSEGEA SGRTDTSRST SGEPKERDKE EGKDSKPRSL RFTWSMKTTS SMDPNDMMRE 720
IRKVLDANNC DYEQKERFLL FCVHGDARQD SLVQWEMEVC KLPRLSLNGV RFKRISGTSI 780
AFKNIASKIA NELKL 795 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0015630; C:microtubule cytoskeleton; TAS:ProtInc.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0070300; F:phosphatidic acid binding; IDA:UniProtKB.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:UniProtKB.
 GO:0001786; F:phosphatidylserine binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0050321; F:tau-protein kinase activity; ISS:UniProtKB.
 GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0001764; P:neuron migration; ISS:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR001772; KA1_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 
Pfam
 PF02149; KA1
 PF00069; Pkinase 
SMART
 SM00220; S_TKc
 SM00165; UBA 
PROSITE
 PS50032; KA1
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50030; UBA 
PRINTS