| Tag | Content |
|---|
| CPLM ID | CPLM-002552 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Guanine nucleotide exchange factor LTE1 |
Protein Synonyms/Alias | Low temperature essential protein 1 |
Gene Name | LTE1 |
Gene Synonyms/Alias | EIS4; MSI2; YAL024C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 268 | YSKHRIAKEPSVDNE | ubiquitination | [1] | | 427 | SLNRKSSKEEEEEFL | ubiquitination | [1] | | 729 | LEKNYDNKENQESEY | ubiquitination | [1] | | 741 | SEYESTKKLDNSLDA | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis. |
Sequence Annotation | DOMAIN 25 157 N-terminal Ras-GEF.
DOMAIN 1194 1434 Ras-GEF.
MOD_RES 271 271 Phosphoserine.
MOD_RES 559 559 Phosphoserine.
MOD_RES 689 689 Phosphoserine.
MOD_RES 691 691 Phosphothreonine.
MOD_RES 808 808 Phosphoserine.
MOD_RES 810 810 Phosphoserine.
MOD_RES 1028 1028 Phosphoserine.
MOD_RES 1109 1109 Phosphoserine. |
Keyword | Cell cycle; Cell division; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Mitosis; Phosphoprotein; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1435 AA |
Protein Sequence | MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY NASADFFLIY 60
RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA LVRTFVLLRH SILNYFVQDF 120
LPNITLRLRL IEFLNDKHIE QYPKIISSCI INLKKNWVHC SKLVWENIEL NEPDKLDFDA 180
WLHYSLKDFT QLESLHKRGS RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN 240
SSKNQRSPSM LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK 300
GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS LDNNSNSNSR 360
SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK PANQKPEMDA FVKYVVSISS 420
LNRKSSKEEE EEFLNSDSSK FDILSARTID EVESLLHLQN QLIEKVQTHS NNNRGPTVNV 480
DCERREHIHD IKILQQNSFK PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE 540
SNMQPSPSYD ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH 600
ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE DTESITYSYD 660
SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN LREFTFEDTK SLDEKKSTID 720
GLEKNYDNKE NQESEYESTK KLDNSLDASS EANNYDITTR KKHSSCNHKI KQAVVRPASG 780
RISISRVQSI AITPTKELSI VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI 840
SISTSKLFES AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS 900
TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS NNDLESPSTH 960
AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS NISGSVLTMD DIDINDTSSA 1020
RNTRRANSES AFTGSLNKKN LNEIANMLDD SINDDPITVA LMKLEGTYEK IPEKPENTKS 1080
SDAIGIKTSK LADEVEMLNL NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG 1140
FTSSSPEKID VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ 1200
QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI SRFNLTVDWI 1260
ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII LALSSSVVQK FTDAWRLIEP 1320
GDLLTWEELK KIPSLDRNYS TIRNLLNSVN PLVGCVPFIV VYLSDLSANA EKKDWILEDK 1380
VVNYNKFDTN VQIVKNFIQR VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST 1435 |
Gene Ontology | GO:0005933; C:cellular bud; IDA:SGD. GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:SGD. GO:0051301; P:cell division; IEA:UniProtKB-KW. GO:0007067; P:mitosis; IEA:UniProtKB-KW. GO:0031578; P:mitotic spindle orientation checkpoint; TAS:SGD. GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. GO:0016192; P:vesicle-mediated transport; IGI:SGD. |
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