UniProt Accession

 PKN2_MOUSE; Q8BWW9; Q3TBR3; Q80WS2; Q8BJL7; Q8BL62 

Genbank Protein ID

 AK046248; AK133298; AK171092; AK049713; AK083425; BC052073 

Genbank Nucleotide ID

 BAC32655.1; BAE21599.1; BAE42244.1; BAC33888.1; BAC38910.1; AAH52073.1 

Protein Name

 Serine/threonine-protein kinase N2 

Protein Synonyms/Alias

 PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2 

Gene Name

 Pkn2 

Gene Synonyms/Alias

 Prk2; Prkcl2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
929	ALMDKKVKPPFVPTI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). 

Sequence Annotation

 REPEAT 44 119 REM 1.
 REPEAT 133 213 REM 2.
 REPEAT 214 295 REM 3.
 DOMAIN 329 462 C2.
 DOMAIN 656 915 Protein kinase.
 DOMAIN 916 983 AGC-kinase C-terminal.
 NP_BIND 662 670 ATP (By similarity).
 REGION 381 462 Necessary to rescue apical junction
 REGION 916 976 Necessary for the catalytic activity (By
 REGION 977 983 Negatively regulates the responsiveness
 ACT_SITE 781 781 Proton acceptor (By similarity).
 BINDING 685 685 ATP (By similarity).
 MOD_RES 110 110 Phosphoserine (By similarity).
 MOD_RES 121 121 Phosphothreonine (By similarity).
 MOD_RES 124 124 Phosphothreonine (By similarity).
 MOD_RES 301 301 Phosphoserine (By similarity).
 MOD_RES 305 305 Phosphoserine (By similarity).
 MOD_RES 359 359 Phosphoserine (By similarity).
 MOD_RES 361 361 Phosphoserine (By similarity).
 MOD_RES 366 366 Phosphoserine.
 MOD_RES 534 534 Phosphoserine (By similarity).
 MOD_RES 582 582 Phosphoserine.
 MOD_RES 630 630 Phosphoserine (By similarity).
 MOD_RES 815 815 Phosphothreonine; by PDPK1 (By
 MOD_RES 957 957 Phosphothreonine (Probable).  

Keyword

 Alternative splicing; Apoptosis; ATP-binding; Cell adhesion; Cell cycle; Cell division; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 983 AA 

Protein Sequence

Gene Ontology

 GO:0043296; C:apical junction complex; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042826; F:histone deacetylase binding; ISS:UniProtKB.
 GO:0004697; F:protein kinase C activity; IEA:EC.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0043297; P:apical junction assembly; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010631; P:epithelial cell migration; ISS:UniProtKB.
 GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; ISS:UniProtKB.
 GO:2000145; P:regulation of cell motility; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR000961; AGC-kinase_C.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR011072; HR1_rho-bd.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF02185; HR1
 PF00069; Pkinase
 PF00433; Pkinase_C 

SMART

 SM00239; C2
 SM00742; Hr1
 SM00133; S_TK_X
 SM00220; S_TKc 

PROSITE

 PS51285; AGC_KINASE_CTER
 PS50004; C2
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS