CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013646
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 28 
Protein Synonyms/Alias
 Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28 
Gene Name
 Usp28 
Gene Synonyms/Alias
 Kiaa1515 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
64LLTDQRVKEPSHDTTubiquitination[1]
426EIQVLQQKLERYVKYubiquitination[1]
547DEEMNFVKTCLQRWRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex (By similarity). 
Sequence Annotation
 REPEAT 97 116 UIM.
 ACT_SITE 171 171 Nucleophile (By similarity).
 ACT_SITE 605 605 Proton acceptor (By similarity).
 MOD_RES 67 67 Phosphoserine (By similarity).
 MOD_RES 720 720 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; DNA damage; DNA repair; Hydrolase; Nucleus; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1051 AA 
Protein Sequence
MTAELQQDDS AGAADGHGSS CQMLLNQLRE ITGIQDPSFL HEALKASNGD ITQAVSLLTD 60
QRVKEPSHDT TAAEPSEVEE SATSKDLLAK VIDLTHDNKD DLQAAIALSL LESPNIQADN 120
RDLNRAHEAN SAETKRSKRK RCEVWGENHN PNNWRRVDGW PVGLKNVGNT CWFSAVIQSL 180
FQLPEFRRLV LSYNLPQNIL ENCRSHTEKR NIMFMQELQY LFALLLGSNR KFVDPSAALD 240
LLKGAFRSSE EQQQDVSEFT HKLLDWLEDA FQLAVNVNSH LRNKSENPMV QLFYGTFLTE 300
GVREGKPFCN NETFGQYPLQ VNGYHNLDEC LEGAMVEGDI ALLPSDRSVK YGQERWFTKL 360
PPVLTFELSR FEFNQSLGQP EKIHNKLEFP QIIYMDRYMY KSKELIRSKR ESVRKLKEEI 420
QVLQQKLERY VKYGSGPSRF PLPDMLKYVI EFASTKPASE SCLSGSAEHV TLPLPSVHCP 480
ISDLTPKESS SPESCSQNAG STFSSPEDAL PSSEGMNGPF TSPHSSLETP APPAPRTVTD 540
EEMNFVKTCL QRWRSEIEQD IQDLKNCISS STKAIEQMYC DPLLRQVPYR LHAVLVHEGQ 600
ASAGHYWAYI YNQPRQTWLK YNDISVTESS WEELERDSYG GLRNVSAYCL MYINDNLPHF 660
SAEASSNESD ETAGEVEALS VELRQYIQED NWRFQQEVEE WEEEQSCKIP QMESSPNSSS 720
QDFSTSQESP AVSSHEVRCL SSEHAVIAKE QTAQAIANTA HAYEKSGVEA ALSEAFHEEY 780
SRLYQLAKET PTSHSDPRLQ HVLVYFFQNE APKRVVERTL LEQFADRNLS YDERSISIMK 840
VAQAKLMEIG PDDMNMEEYK RWHEDYSLFR KVSVYLLTGL ELFQKGKYQE ALSYLVYAYQ 900
SNAGLLVKGP RRGVKESVIA LYRRKCLLEL NAKAASLFET NDDHSVTEGI NVMNELIIPC 960
IHLIINNDIS KDDLDAIEVM RNHWCSYLGK DIAENLQLCL GEFLPRLLDP SAEIIVLKEP 1020
PTIRPNSPYD LCNRFAAVME SIQGVSTVTV K 1051 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005654; C:nucleoplasm; ISS:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; ISS:UniProtKB.
 GO:0034644; P:cellular response to UV; IEA:Compara.
 GO:0000077; P:DNA damage checkpoint; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR009060; UBA-like.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF00443; UCH 
SMART
 SM00726; UIM 
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3
 PS50330; UIM 
PRINTS