| Tag | Content |
|---|
| CPLM ID | CPLM-007231 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Golgi apyrase |
Protein Synonyms/Alias | ATP-diphosphatase; ATP-diphosphohydrolase; Adenosine diphosphatase; ADPase; Golgi nucleoside diphosphatase; Yeast nucleoside diphosphatase 1 |
Gene Name | YND1 |
Gene Synonyms/Alias | YER005W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 570 | GTDVDGFKDVYRMKS | ubiquitination | [1] | | 576 | FKDVYRMKSSSMFDL | ubiquitination | [1] | | 585 | SSMFDLGKSSATMQR | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Has equal high activity toward ADP/ATP, GDP/GTP, and UDP/UTP and approximately 50% less toward CDP/CTP and thiamine pyrophosphate. Has no activity toward GMP. Required for Golgi glycosylation and cell wall integrity. Together with CDC55, required for adenovirus E4orf4 (early region 4 open reading frame 4) induced toxicity, the apyrase activity is not required for this function. Plays a role in sphingolipid synthesis. |
Sequence Annotation | ACT_SITE 152 152 Proton acceptor (By similarity). |
Keyword | ATP-binding; Complete proteome; Golgi apparatus; Hydrolase; Lipid metabolism; Membrane; Nucleotide-binding; Reference proteome; Sphingolipid metabolism; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 630 AA |
Protein Sequence | MLIENTNDRF GIVIDAGSSG SRIHVFKWQD TESLLHATNQ DSQSILQSVP HIHQEKDWTF 60
KLNPGLSSFE KKPQDAYKSH IKPLLDFAKN IIPESHWSSC PVFIQATAGM RLLPQDIQSS 120
ILDGLCQGLK HPAEFLVEDC SAQIQVIDGE TEGLYGWLGL NYLYGHFNDY NPEVSDHFTF 180
GFMDMGGAST QIAFAPHDSG EIARHRDDIA TIFLRSVNGD LQKWDVFVST WLGFGANQAR 240
RRYLAQLINT LPENTNDYEN DDFSTRNLND PCMPRGSSTD FEFKDTIFHI AGSGNYEQCT 300
KSIYPLLLKN MPCDDEPCLF NGVHAPRIDF ANDKFIGTSE YWYTANDVFK LGGEYNFDKF 360
SKSLREFCNS NWTQILANSD KGVYNSIPEN FLKDACFKGN WVLNILHEGF DMPRIDVDAE 420
NVNDRPLFQS VEKVEERELS WTLGRILLYA SGSILAGNDD FMVGIAPSER RTKLTGKKFI 480
PGKLLESDQL RKQSSSLSNK GFLMWFAIIC CIFYLIFHRS HIIRRRFSGL YNITKDFKTG 540
IRRRLKFLRR SDPFSRLEEG ELGTDVDGFK DVYRMKSSSM FDLGKSSATM QREHEPQRTA 600
SQSANLAPSN LRPAFSMADF SKFKDSRLYD 630 |
Gene Ontology | GO:0000139; C:Golgi membrane; IDA:SGD. GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0017110; F:nucleoside-diphosphatase activity; IDA:SGD. GO:0017111; F:nucleoside-triphosphatase activity; IDA:SGD. GO:0006486; P:protein glycosylation; IMP:SGD. GO:0006665; P:sphingolipid metabolic process; IEA:UniProtKB-KW. |
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