CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043236
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD8 ultimate buster 1 
Protein Synonyms/Alias
 NEDD8 ultimate buster-1, isoform CRA_d 
Gene Name
 NUB1 
Gene Synonyms/Alias
 NYREN18; hCG_19587 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34QKKYLQAKLTQFLREubiquitination[1]
339NLAQKCFKNCYGENHubiquitination[2]
353HQRLVHIKGNCGKEKubiquitination[2, 3]
389EAYEYLNKARQLFKEubiquitination[2, 3]
395NKARQLFKELYIDPSubiquitination[1, 2, 4, 5, 6]
403ELYIDPSKVDNLLQLubiquitination[6]
464LENIRFLKGMGYSTHubiquitination[6]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 625 AA 
Protein Sequence
MKTNGGRCRI RALCWSRREW RGAGMAQKKY LQAKLTQFLR EDRIQLWKPP YTDENKKVGL 60
ALKDLAKQYS DRLECCENEV EKVIEEIRCK AIERGTGNDN YRTTGIATIE VFLPPRLKKD 120
RKNLLETRLH ITGRELRSKI AETFGLQENY IKIVINKKQL QLGKTLEEQG VAHNVKAMVL 180
ELKQSEEDAR KNFQLEEEEQ NEAKLKEKQI QRTKRGLEIL AKRAAETVVD PEMTPYLDIA 240
NQTGRSIRIP PSERKALMLA MGYHEKGRAF LKRKEYGIAL PCLLDADKYF CECCRELLDT 300
VDNYAVLQLD IVWCYFRLEQ LECLDDAEKK LNLAQKCFKN CYGENHQRLV HIKGNCGKEK 360
VLFLRLYLLQ GIRNYHSGND VEAYEYLNKA RQLFKELYID PSKVDNLLQL GFTAQEARLG 420
LRACDGNVDH AATHITNRRE ELAQIRKEEK EKKRRRLENI RFLKGMGYST HAAQQILLSN 480
PQMWWLNDSN PETDNRQESP SQENIDRLVY MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG 540
GSLPPELPLS PEDSLSPPAT SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST 600
LEDEEIIIAE YLSYVENRKS ATKKN 625 
Gene Ontology
  
Interpro
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 
Pfam
 PF00627; UBA 
SMART
 SM00165; UBA 
PROSITE
 PS50030; UBA 
PRINTS