CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000958
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear export mediator factor NEMF 
Protein Synonyms/Alias
 Antigen NY-CO-1; Serologically defined colon cancer antigen 1 
Gene Name
 NEMF 
Gene Synonyms/Alias
 SDCCAG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35NVYDVDNKTYLIRLQubiquitination[1, 2, 3, 4]
311EGQKIDLKALQQEKQubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a role in nuclear export. 
Sequence Annotation
 MOD_RES 417 417 Phosphoserine.
 MOD_RES 747 747 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.
 MOD_RES 763 763 Phosphoserine.
 MOD_RES 831 831 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1076 AA 
Protein Sequence
MKSRFSTIDL RAVLAELNAS LLGMRVNNVY DVDNKTYLIR LQKPDFKATL LLESGIRIHT 60
TEFEWPKNMM PSSFAMKCRK HLKSRRLVSA KQLGVDRIVD FQFGSDEAAY HLIIELYDRG 120
NIVLTDYEYV ILNILRFRTD EADDVKFAVR ERYPLDHARA AEPLLTLERL TEIVASAPKG 180
ELLKRVLNPL LPYGPALIEH CLLENGFSGN VKVDEKLETK DIEKVLVSLQ KAEDYMKTTS 240
NFSGKGYIIQ KREIKPSLEA DKPVEDILTY EEFHPFLFSQ HSQCPYIEFE SFDKAVDEFY 300
SKIEGQKIDL KALQQEKQAL KKLDNVRKDH ENRLEALQQA QEIDKLKGEL IEMNLQIVDR 360
AIQVVRSALA NQIDWTEIGL IVKEAQAQGD PVASAIKELK LQTNHVTMLL RNPYLLSEEE 420
DDDVDGDVNV EKNETEPPKG KKKKQKNKQL QKPQKNKPLL VDVDLSLSAY ANAKKYYDHK 480
RYAAKKTQKT VEAAEKAFKS AEKKTKQTLK EVQTVTSIQK ARKVYWFEKF LWFISSENYL 540
IIGGRDQQQN EIIVKRYLTP GDIYVHADLH GATSCVIKNP TGEPIPPRTL TEAGTMALCY 600
SAAWDARVIT SAWWVYHHQV SKTAPTGEYL TTGSFMIRGK KNFLPPSYLM MGFSFLFKVD 660
ESCVWRHQGE RKVRVQDEDM ETLASCTSEL ISEEMEQLDG GDTSSDEDKE EHETPVEVEL 720
MTQVDQEDIT LQSGRDELNE ELIQEESSED EGEYEEVRKD QDSVGEMKDE GEETLNYPDT 780
TIDLSHLQPQ RSIQKLASKE ESSNSSDSKS QSRRHLSAKE RREMKKKKLP SDSGDLEALE 840
GKDKEKESTV HIETHQNTSK NVAAVQPMKR GQKSKMKKMK EKYKDQDEED RELIMKLLGS 900
AGSNKEEKGK KGKKGKTKDE PVKKQPQKPR GGQRVSDNIK KETPFLEVIT HELQDFAVDD 960
PHDDKEEQDL DQQGNEENLF DSLTGQPHPE DVLLFAIPIC APYTTMTNYK YKVKLTPGVQ 1020
KKGKAAKTAL NSFMHSKEAT AREKDLFRSV KDTDLSRNIP GKVKVSAPNL LNVKRK 1076 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0051168; P:nuclear export; IMP:UniProtKB. 
Interpro
 IPR021846; DUF3441.
 IPR008532; DUF814.
 IPR008616; Fibro-bd_N. 
Pfam
 PF11923; DUF3441
 PF05670; DUF814
 PF05833; FbpA 
SMART
  
PROSITE
  
PRINTS