CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007205
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase RSP5 
Protein Synonyms/Alias
 Reverses SPT-phenotype protein 5 
Gene Name
 RSP5 
Gene Synonyms/Alias
 MDP1; NPI1; YER125W; SYGP-ORF41 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
45KSTSAAKKTLNPYWNacetylation[1]
45KSTSAAKKTLNPYWNubiquitination[2]
78FDQKKFKKKDQGFLGubiquitination[3]
79DQKKFKKKDQGFLGVubiquitination[2, 3]
258NTRTTTWKRPTLDQTubiquitination[2, 3]
411YFVDHNTKTTTWDDPubiquitination[2]
432DQNVPQYKRDFRRKVubiquitination[2]
640IEVTDGNKKEYVELYubiquitination[2]
773GEVQQLPKSHTCFNRubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o- dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner. 
Sequence Annotation
 DOMAIN 1 88 C2.
 DOMAIN 229 262 WW 1.
 DOMAIN 331 364 WW 2.
 DOMAIN 387 420 WW 3.
 DOMAIN 705 809 HECT.
 ACT_SITE 777 777 Glycyl thioester intermediate.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Cytoskeleton; Ligase; Nucleus; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 809 AA 
Protein Sequence
MPSSISVKLV AAESLYKRDV FRSPDPFAVL TIDGYQTKST SAAKKTLNPY WNETFKFDDI 60
NENSILTIQV FDQKKFKKKD QGFLGVVNVR VGDVLGHLDE DTATSSGRPR EETITRDLKK 120
SNDGMAVSGR LIVVLSKLPS SSPHSQAPSG HTASSSTNTS STTRTNGHST SSTRNHSTSH 180
PSRGTAQAVE STLQSGTTAA TNTATTSHRS TNSTSSATRQ YSSFEDQYGR LPPGWERRTD 240
NFGRTYYVDH NTRTTTWKRP TLDQTEAERG NQLNANTELE RRQHRGRTLP GGSSDNSSVT 300
VQVGGGSNIP PVNGAAAAAF AATGGTTSGL GELPSGWEQR FTPEGRAYFV DHNTRTTTWV 360
DPRRQQYIRT YGPTNTTIQQ QPVSQLGPLP SGWEMRLTNT ARVYFVDHNT KTTTWDDPRL 420
PSSLDQNVPQ YKRDFRRKVI YFRSQPALRI LPGQCHIKVR RKNIFEDAYQ EIMRQTPEDL 480
KKRLMIKFDG EEGLDYGGVS REFFFLLSHE MFNPFYCLFE YSAYDNYTIQ INPNSGINPE 540
HLNYFKFIGR VVGLGVFHRR FLDAFFVGAL YKMMLRKKVV LQDMEGVDAE VYNSLNWMLE 600
NSIDGVLDLT FSADDERFGE VVTVDLKPDG RNIEVTDGNK KEYVELYTQW RIVDRVQEQF 660
KAFMDGFNEL IPEDLVTVFD ERELELLIGG IAEIDIEDWK KHTDYRGYQE SDEVIQWFWK 720
CVSEWDNEQR ARLLQFTTGT SRIPVNGFKD LQGSDGPRRF TIEKAGEVQQ LPKSHTCFNR 780
VDLPQYVDYD SMKQKLTLAV EETIGFGQE 809 
Gene Ontology
 GO:0030479; C:actin cortical patch; IEA:UniProtKB-SubCell.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0010008; C:endosome membrane; IDA:SGD.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:SGD.
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0000151; C:ubiquitin ligase complex; IDA:MGI.
 GO:0035091; F:phosphatidylinositol binding; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:MGI.
 GO:0034644; P:cellular response to UV; IMP:SGD.
 GO:0006333; P:chromatin assembly or disassembly; IMP:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
 GO:0007005; P:mitochondrion organization; IMP:SGD.
 GO:0045723; P:positive regulation of fatty acid biosynthetic process; IMP:SGD.
 GO:0048260; P:positive regulation of receptor-mediated endocytosis; IMP:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IPI:SGD.
 GO:0006513; P:protein monoubiquitination; IDA:SGD.
 GO:0000209; P:protein polyubiquitination; IDA:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0032956; P:regulation of actin cytoskeleton organization; IGI:SGD.
 GO:0010794; P:regulation of dolichol biosynthetic process; IMP:SGD.
 GO:0032443; P:regulation of ergosterol biosynthetic process; IMP:SGD.
 GO:0010793; P:regulation of mRNA export from nucleus; IMP:SGD.
 GO:0010796; P:regulation of multivesicular body size; IMP:SGD.
 GO:0006808; P:regulation of nitrogen utilization; IGI:SGD.
 GO:0019220; P:regulation of phosphate metabolic process; IGI:SGD.
 GO:0032880; P:regulation of protein localization; IMP:SGD.
 GO:2000203; P:regulation of ribosomal large subunit export from nucleus; IMP:SGD.
 GO:2000232; P:regulation of rRNA processing; IMP:SGD.
 GO:2000238; P:regulation of tRNA export from nucleus; IMP:SGD.
 GO:2000235; P:regulation of tRNA processing; IMP:SGD.
 GO:0010795; P:regulation of ubiquinone biosynthetic process; IMP:SGD.
 GO:0042493; P:response to drug; IMP:SGD.
 GO:0034517; P:ribophagy; IGI:SGD.
 GO:0070086; P:ubiquitin-dependent endocytosis; IMP:SGD.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR024928; E3_ub_ligase_SMURF1.
 IPR000569; HECT.
 IPR001202; WW_dom. 
Pfam
 PF00168; C2
 PF00632; HECT
 PF00397; WW 
SMART
 SM00239; C2
 SM00119; HECTc
 SM00456; WW 
PROSITE
 PS50004; C2
 PS50237; HECT
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS