CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011635
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial 
Protein Synonyms/Alias
 ETF-QO; ETF-ubiquinone oxidoreductase; Changed intracellular redox state protein 2; Electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase 
Gene Name
 CIR2 
Gene Synonyms/Alias
 YOR356W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
51ANLTEEEKELLNEPRacetylation[1]
335NPYVSPYKEFQKMKHacetylation[1]
471IRPSFSGKLGGYGGMubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Accepts electrons from ETF and reduces ubiquinone (By similarity). 
Sequence Annotation
 DOMAIN 591 620 4Fe-4S ferredoxin-type.
 NP_BIND 65 79 FAD (Potential).
 METAL 574 574 Iron-sulfur (4Fe-4S) (Potential).
 METAL 600 600 Iron-sulfur (4Fe-4S) (Potential).
 METAL 603 603 Iron-sulfur (4Fe-4S) (Potential).
 METAL 606 606 Iron-sulfur (4Fe-4S) (Potential).  
Keyword
 4Fe-4S; Complete proteome; Electron transport; FAD; Flavoprotein; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Reference proteome; Transit peptide; Transport; Ubiquinone. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 631 AA 
Protein Sequence
MIKFTNENLI RGIRMTISAK SRHLALGTDM TRKFSLSCRF LNKANLTEEE KELLNEPRAR 60
DYVDVCIVGG GPAGLATAIK LKQLDNSSGT GQLRVVVLEK SSVLGGQTVS GAILEPGVWK 120
ELFPDEKSDI GIPLPKELAT LVTKEHLKFL KGKWAISVPE PSQMINKGRN YIVSLNQVVG 180
YLGEKAEEVG VEVYPGIAVS DLIYDENNAV KGVITKDAGI SKSGKPKETF ERGMEFWARQ 240
TVLAEGCHGS LTKQALAKYD LRKGRQHQTY GLGIKEVWEV KPENFNKGFA AHTMGYPLTN 300
DVYGGGFQYH FGDGLVTVGL VVGLDYKNPY VSPYKEFQKM KHHPYYSKVL EGGKCIAYAA 360
RALNEGGLQS VPKLNFPGGV LVGASAGFMN VPKIKGTHTA MKSGLLAAES IFESIKGLPV 420
LEEVEDEDAK MAMFDKEATI NLESYESAFK ESSIYKELYE VRNIRPSFSG KLGGYGGMIY 480
SGIDSLILKG KVPWTLKFDE KNDGEILEPA SKYKPIEYPK PDGVISFDIL TSVSRTGTYH 540
DDDEPCHLRV PGQDMVKYAE RSFPVWKGVE SRFCPAGVYE FVKDEKSPVG TRLQINSQNC 600
IHCKTCDIKA PRQDITWKVP EGGDGPKYTL T 631 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 
Interpro
 IPR017896; 4Fe4S_Fe-S-bd.
 IPR007859; ETFD_OxRdtase.
 IPR006076; FAD-dep_OxRdtase. 
Pfam
 PF01266; DAO
 PF05187; ETF_QO 
SMART
  
PROSITE
 PS51379; 4FE4S_FER_2 
PRINTS