UniProt Accession

 RAN_HUMAN; P62826; A8K3Z8; P17080; P28746; P28747; Q6IPB2; Q86V08; Q8NI90; Q9CSP3; Q9CWI7; Q9CZA2; Q9UDJ5; Q9UEU9 

Genbank Protein ID

 M31469; AF052578; AF054183; AF501887; BT007271; CR450347; AK290763; AK312466; CH471054; BC004272; BC014518; BC014901; BC016654; BC051908; BC072000; AB062399 

Genbank Nucleotide ID

 AAA36546.1; AAC05840.1; AAC99400.1; AAM15923.1; AAP35935.1; CAG29343.1; BAF83452.1; BAG35373.1; EAW98516.1; AAH04272.3; AAH14518.1; AAH14901.1; AAH16654.1; AAH51908.2; AAH72000.1; BAB93486.1 

Protein Name

 GTP-binding nuclear protein Ran 

Protein Synonyms/Alias

 Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein 

Gene Name

 RAN 

Gene Synonyms/Alias

 ARA24; OK/SW-cl.81 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
23	VGDGGTGKTTFVKRH	ubiquitination	[1]
37	HLTGEFEKKYVATLG	acetylation	[2]
38	LTGEFEKKYVATLGV	ubiquitination	[1]
60	HTNRGPIKFNVWDTA	acetylation	[2]
60	HTNRGPIKFNVWDTA	ubiquitination	[1, 3, 4]
71	WDTAGQEKFGGLRDG	acetylation	[2]
71	WDTAGQEKFGGLRDG	ubiquitination	[1, 3, 4, 5, 6]
99	VTSRVTYKNVPNWHR	acetylation	[2]
99	VTSRVTYKNVPNWHR	ubiquitination	[1, 3, 4]
134	KDRKVKAKSIVFHRK	ubiquitination	[1, 4]
142	SIVFHRKKNLQYYDI	ubiquitination	[1, 3, 4]
152	QYYDISAKSNYNFEK	ubiquitination	[1, 4]
159	KSNYNFEKPFLWLAR	acetylation	[2]
159	KSNYNFEKPFLWLAR	ubiquitination	[1, 3, 4, 6, 7]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
 Meierhofer D, Wang X, Huang L, Kaiser P.
 J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 

Functional Description

 GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. 

Sequence Annotation

 NP_BIND 17 24 GTP (By similarity).
 NP_BIND 65 69 GTP (By similarity).
 NP_BIND 122 125 GTP (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 60 60 N6-acetyllysine.
 MOD_RES 71 71 N6-acetyllysine; alternate.
 MOD_RES 99 99 N6-acetyllysine.
 MOD_RES 159 159 N6-acetyllysine.
 CROSSLNK 71 71 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Cell cycle; Cell division; Complete proteome; Cytoplasm; Direct protein sequencing; GTP-binding; Host-virus interaction; Isopeptide bond; Mitosis; Nucleotide-binding; Nucleus; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 216 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005643; C:nuclear pore; NAS:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; TAS:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; TAS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0034629; P:cellular protein complex localization; IEA:Compara.
 GO:0006259; P:DNA metabolic process; TAS:UniProtKB.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0075733; P:intracellular transport of viral material; TAS:Reactome.
 GO:0007067; P:mitosis; TAS:UniProtKB.
 GO:0007052; P:mitotic spindle organization; TAS:UniProtKB.
 GO:0032092; P:positive regulation of protein binding; IDA:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006611; P:protein export from nucleus; NAS:UniProtKB.
 GO:0006606; P:protein import into nucleus; IEA:Compara.
 GO:0006405; P:RNA export from nucleus; NAS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0019058; P:viral infectious cycle; TAS:Reactome. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR002041; Ran_GTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase. 

Pfam

 PF00071; Ras 

SMART

 SM00176; RAN 

PROSITE

 PS51418; RAN 

PRINTS

 PR00627; GTPRANTC4.