CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010041
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate-protein phosphotransferase 
Protein Synonyms/Alias
 Phosphotransferase system, enzyme I 
Gene Name
 ptsI 
Gene Synonyms/Alias
 MPN_627; MP215 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
30TPEFAVNKYLKHQLTacetylation[1]
146LAYLTGQKPHDLVKIacetylation[1]
207IPAVVGIKGITTKVKacetylation[1]
241SKDTTEWKKQKALEEacetylation[1]
249KQKALEEKYQQELKQacetylation[1]
559ANAKEVEKLVEKFFKacetylation[1]
563EVEKLVEKFFKKKDIacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819
Functional Description
 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity). 
Sequence Annotation
 ACT_SITE 190 190 Tele-phosphohistidine intermediate (By
 ACT_SITE 498 498 Proton donor (By similarity).
 METAL 427 427 Magnesium (By similarity).
 METAL 451 451 Magnesium (By similarity).
 BINDING 297 297 Substrate (By similarity).
 BINDING 333 333 Substrate (By similarity).
 BINDING 427 427 Substrate (By similarity).
 BINDING 448 448 Substrate; via carbonyl oxygen (By
 BINDING 449 449 Substrate; via amide nitrogen (By
 BINDING 450 450 Substrate (By similarity).
 BINDING 451 451 Substrate; via amide nitrogen (By  
Keyword
 Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MKKLSGIGVS DGMALAKAFL VKTPEFAVNK YLKHQLTKAQ AKRLLDSAFK KAVKDLEEIK 60
EITVNNINTE AGMIFDAHIQ MLNDPTITEQ LEQQLAQNVH PVIAVDTVFS QTATMFSQMQ 120
DKYFQERAAD ILDLRQRLLA YLTGQKPHDL VKIKSDVIIV AHDLTPSQTA TLNKKYVKGF 180
LTEIGGRTSH AAIMARSLEI PAVVGIKGIT TKVKDGQIVG VDGRKGIAGL DLNSKDTTEW 240
KKQKALEEKY QQELKQYTNK ETVTLDGHAV VVAANIGNVK DMELACQYNT NGVGLFRTEF 300
LYMNSQEWPD EETQYQAYKA VLEQAHGDLV IIRTLDIGGD KKLNYYEFPH EDNPFLGYRA 360
LRLTLDKQDI FKTQLRALLR AADHGQLGIM FPMVATLDEL LQAKQLLNQV HQELGGNKQF 420
KLGIMIEIPA AVLAANTLSH HVDFFSIGTN DLIQYSFAAD RMNKNVSYLY QPLNPALLKL 480
IYLTIEGGKV NDIWTGMCGE MAGEPLAIPL LLGLGLKEFS MSASSMFKAR MIIAKLNYTE 540
CQTLAQKALT LANAKEVEKL VEKFFKKKDI FI 572 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:EC.
 GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
 GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. 
Interpro
 IPR008279; PEP-util_enz_mobile_dom.
 IPR018274; PEP_util_AS.
 IPR000121; PEP_util_C.
 IPR023151; PEP_util_CS.
 IPR006318; PEP_util_enz.
 IPR024692; PTS_enz_I.
 IPR008731; PTS_PEP_utilis_N.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. 
Pfam
 PF05524; PEP-utilisers_N
 PF00391; PEP-utilizers
 PF02896; PEP-utilizers_C 
SMART
  
PROSITE
 PS00742; PEP_ENZYMES_2
 PS00370; PEP_ENZYMES_PHOS_SITE 
PRINTS
 PR01736; PHPHTRNFRASE.