UniProt Accession

 GET3_YEAST; Q12154; D6VRQ0; Q6B1B6 

Genbank Protein ID

 X95644; Z74148; AY693164; BK006938 

Genbank Nucleotide ID

 CAA64913.1; CAA98667.1; AAT93183.1; DAA11760.1 

Protein Name

 ATPase GET3 

Protein Synonyms/Alias

 Arsenical pump-driving ATPase; Arsenite-stimulated ATPase; Golgi to ER traffic protein 3; Guided entry of tail-anchored proteins 3 

Gene Name

 GET3 

Gene Synonyms/Alias

 ARR4; YDL100C; D2371 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
26	KWIFVGGKGGVGKTT	ubiquitination	[1]
189	TLSKLLEKFGEITNK	acetylation	[2]
297	ARWKMQKKYLDQIDE	ubiquitination	[1]
313	YEDFHVVKMPLCAGE	ubiquitination	[1]
329	RGLNNLTKFSQFLNK	ubiquitination	[1]
345	YNPITDGKVIYELED	acetylation	[2]
353	VIYELEDKE******	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. 

Sequence Annotation

 NP_BIND 26 33 ATP.
 NP_BIND 315 322 ATP.
 ACT_SITE 57 57
 METAL 285 285 Zinc; shared with dimeric partner.
 METAL 288 288 Zinc; shared with dimeric partner.
 BINDING 245 245 ATP.
 BINDING 272 272 ATP.  

Keyword

 3D-structure; Arsenical resistance; ATP-binding; Complete proteome; Cytoplasm; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Hydrolase; Metal-binding; Nucleotide-binding; Reference proteome; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 354 AA 

Protein Sequence

Gene Ontology

 GO:0043529; C:GET complex; IPI:SGD.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
 GO:0006620; P:posttranslational protein targeting to membrane; IDA:SGD.
 GO:0045048; P:protein insertion into ER membrane; IMP:SGD.
 GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
 GO:0009408; P:response to heat; IMP:SGD.
 GO:0010038; P:response to metal ion; IMP:SGD.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD. 

Interpro

 IPR025723; Anion-transp_ATPase-like_dom.
 IPR016300; ATPase_ArsA/GET3.
 IPR027542; ATPase_ArsA/GET3_euk.
 IPR027417; P-loop_NTPase. 

Pfam

 PF02374; ArsA_ATPase 

SMART

  

PROSITE

  

PRINTS