CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033851
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Isobutyryl-CoA dehydrogenase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Acad8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
48EEQKGFQKVAFDFAAacetylation[1, 2, 3]
48EEQKGFQKVAFDFAAsuccinylation[2]
68NMAEWDQKELFPVDVacetylation[1, 3, 4, 5]
220ISCIVVEKGTPGLSFacetylation[3]
229TPGLSFGKKEKKVGWacetylation[1]
269QGFLIAMKGLNGGRIacetylation[2, 3]
269QGFLIAMKGLNGGRIsuccinylation[2]
301QEHLKVRKQFGAPLAacetylation[2]
301QEHLKVRKQFGAPLAsuccinylation[2]
301QEHLKVRKQFGAPLAubiquitination[6]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; FAD; Flavoprotein; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MAMLRSGYRR FGCLRAALKS LAQTHHRSIT FCIDPSLGLN EEQKGFQKVA FDFAAREMAP 60
NMAEWDQKEL FPVDVMRKAA QLGFGGVYVR TDVGGSGLSR LDTSVIFEAL ATGCTSTTAY 120
ISIHNMCAWM IDSFGNEEQR HKFCPPLCTM EKFASYCLTE PGAGSDAASL LTSAKQQGDH 180
YILNGSKAFI SGGGESDIYV VMCRTGESGA KGISCIVVEK GTPGLSFGKK EKKVGWNSQP 240
TRAVIFEDCA VPVANRIGTE GQGFLIAMKG LNGGRINVAS CSLGAAHASV ILTQEHLKVR 300
KQFGAPLARS QYLQFQLADM ATKLVASRLM IRTAAVALQE EREDAVALCS MAKLFATEEC 360
FAICNQALQM HGGYGYLKDY AVQQYMRDSR VHQILEGSNE VMRMLISRNL LQD 413 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. 
Interpro
 IPR006089; Acyl-CoA_DH_CS.
 IPR006092; Acyl-CoA_DH_N.
 IPR006090; Acyl-CoA_Oxase/DH_1.
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 
Pfam
 PF00441; Acyl-CoA_dh_1
 PF02770; Acyl-CoA_dh_M
 PF02771; Acyl-CoA_dh_N 
SMART
  
PROSITE
 PS00072; ACYL_COA_DH_1 
PRINTS