CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043042
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase RNF31 
Protein Synonyms/Alias
  
Gene Name
 RNF31 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
231CDRPRGCKGLGLGTEubiquitination[1, 2, 3, 4, 5, 6]
355PYASSLEKGPPKPGPubiquitination[1]
359SLEKGPPKPGPPRRLubiquitination[1, 4, 7]
379SSCGDPEKQRQDKMRubiquitination[4, 6]
384PEKQRQDKMREEGLQubiquitination[4]
424CVRTRRRKVQELQSLubiquitination[4, 5, 6, 7]
485PSWDGPDKQSLVRRLubiquitination[1, 2, 4, 5, 6, 7]
580QHFTIALKEKHITDMubiquitination[4]
628DAYALFHKKLTEGVLubiquitination[7]
629AYALFHKKLTEGVLMubiquitination[4, 6, 7]
693CEDFQNWKRMNDPEYubiquitination[4]
718ENGIDCPKCKFSYALubiquitination[4]
755NAFYAKNKCPEPNCRubiquitination[4]
789WTALRLQKLLQDNNVubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 917 AA 
Protein Sequence
XNNPVFRSTV DAVQGGRDVL RLYGYTEEQP DGLSFPEGQE EPDEHQVATV TLEVLLLRTE 60
LSLLLQNTHP RQQALEQLLE DKVEDDMLQL SEFDPLLREI APGPLTTPSV PGSTPGPCFL 120
CGSAPGTLHC PSCKQALCPA CDHLFHGHPS RAHHLRQTLP GVLQGTHLSP SLPASAQPRP 180
QSTSLLALGD SSLSSPNPAS AHLPWHCAAC AMLNEPWAVL CVACDRPRGC KGLGLGTEGP 240
QGTGGLEPDL ARGRWACQSC TFENEAAAVL CSICERPRLA QPPSLVVDSR DAGICLQPLQ 300
QGDALLASAQ SQVWYCIHCT FCNSSPGWVC VMCNRTSSPI PAQHAPRPYA SSLEKGPPKP 360
GPPRRLSAPL PSSCGDPEKQ RQDKMREEGL QLVSMIREAR RAWLDRHGNL DEAVEECVRT 420
RRRKVQELQS LGFGPEEGSL QALFQHGGDV SRALTELQRQ RLEPFRQRLW DSGPEPTPSW 480
DGPDKQSLVR RLLAVYALPS WGRAELALSL LQETPRNYEL GDVVEAVRHS QDRAFLRRLL 540
AQECAVCGWA LPHNRMQALT SCECTICPDC FRQHFTIALK EKHITDMVCP ACGRPDLTDD 600
TQLLSYFSTL DIQLRESLEP DAYALFHKKL TEGVLMRDPK FLWCAQCSFG FIYEREQLEA 660
TCPQCHQTFC VRCKRQWEEQ HRGRSCEDFQ NWKRMNDPEY QAQGLAMYLQ ENGIDCPKCK 720
FSYALARGGC MHFHCTQCRH QFCSGCYNAF YAKNKCPEPN CRVKKSLHGH HPRDCLFYLR 780
DWTALRLQKL LQDNNVMFNT EPPAGARAVP GGGCRVIEQK EVPNGLRDEA CGKETPAGYA 840
GLCQAHYKEY LVSLINAHSL DPATLYEVEE LETATERYLH VRPQPLAGED PPAYQARLLQ 900
KLTEEVPLGQ SIPRRRK 917 
Gene Ontology
 GO:0071797; C:LUBAC complex; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 
Interpro
 IPR018997; PUB_domain.
 IPR026254; RNF31.
 IPR009060; UBA-like.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR002867; Znf_C6HC.
 IPR001876; Znf_RanBP2.
 IPR001841; Znf_RING.
 IPR017907; Znf_RING_CS. 
Pfam
 PF01485; IBR
 PF09409; PUB
 PF00641; zf-RanBP 
SMART
 SM00647; IBR
 SM00184; RING
 SM00547; ZnF_RBZ 
PROSITE
 PS50030; UBA
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2
 PS00518; ZF_RING_1 
PRINTS