CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030889
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial 
Protein Synonyms/Alias
 cDNA FLJ60586, highly similar to NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC 1.6.5.3) 
Gene Name
 NDUFS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47GKRAVEDKNIGPLVKubiquitination[1]
173NEEWISDKTRFAYDGubiquitination[1, 2]
182RFAYDGLKRQRLTEPubiquitination[1, 2, 3, 4]
195EPMVRNEKGLLTYTSubiquitination[1, 2, 3]
304LFNARIRKSWLHNDLubiquitination[1]
351HPFSQVLKEAKKPMVubiquitination[1, 2]
383AVSSIAQKIRMTSGVubiquitination[1, 2]
415AALDLGYKPGVEAIRubiquitination[1, 2]
491EGRAQQTKVAVTPPGubiquitination[1, 2, 3]
505GLAREDWKIIRALSEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Ubiquinone. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 611 AA 
Protein Sequence
MEFLLANHPL DCPICDQGGE CDLQDQSMMF GNDRSRFLEG KRAVEDKNIG PLVKTIMTRC 60
IQCTRCIRFA SEIAGVDDLG TTGRGNDMQV GTYIEKMFMS ELSGNIIDIC PVGALTSKPY 120
AFTARPWETR KTESIDVMDA VGSNIVVSTR TGEVMRILPR MHEDINEEWI SDKTRFAYDG 180
LKRQRLTEPM VRNEKGLLTY TSWEDALSRV AGMLQSFQGK DVAAIAGGLV DAEALVALKD 240
LLNRVDSDTL CTEEVFPTAG AGTDLRSNYL LNTTIAGVEE ADVVLLVGTN PRFEAPLFNA 300
RIRKSWLHND LKVALIGSPV DLTYTYDHLG DSPKILQDIA SGSHPFSQVL KEAKKPMVVL 360
GSSALQRNDG AAILAAVSSI AQKIRMTSGV TGDWKVMNIL HRIASQVAAL DLGYKPGVEA 420
IRKNPPKVLF LLGADGGCIT RQDLPKDCFI IYQGHHGDVG APIADVILPG AAYTEKSATY 480
VNTEGRAQQT KVAVTPPGLA REDWKIIRAL SEIAGMTLPY DTLDQVRNRL EEVSPNLVRY 540
DDIEGANYFQ QANELSKLVN QQLLADPLVP PQLTIKDFYM TDSISRASQT MAKCVKAVTE 600
GAQAVEEPSI C 611 
Gene Ontology
 GO:0016020; C:membrane; IEA:InterPro.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
 GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro. 
Interpro
 IPR006656; Mopterin_OxRdtase.
 IPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS.
 IPR010228; NADH_UbQ_OxRdtase_Gsu.
 IPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
 IPR015405; NuoG_C. 
Pfam
 PF09326; DUF1982
 PF00384; Molybdopterin
 PF10588; NADH-G_4Fe-4S_3 
SMART
 SM00929; NADH-G_4Fe-4S_3 
PROSITE
 PS00642; COMPLEX1_75K_2
 PS00643; COMPLEX1_75K_3 
PRINTS