UniProt Accession

 FBRL_MOUSE; P35550; Q99L58 

Genbank Protein ID

 Z22593; BC003813; BC092274 

Genbank Nucleotide ID

 CAA80307.1; AAH03813.1; AAH92274.1 

Protein Name

 rRNA 2'-O-methyltransferase fibrillarin 

Protein Synonyms/Alias

 Nucleolar protein 1 

Gene Name

 Fbl 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
108	GVFICRGKEDALVTK	acetylation	[1]
115	KEDALVTKNLVPGES	ubiquitination	[2]
127	GESVYGEKRVSISEG	acetylation	[3]
127	GESVYGEKRVSISEG	ubiquitination	[2]
137	SISEGDDKIEYRAWN	acetylation	[1]
137	SISEGDDKIEYRAWN	ubiquitination	[2]
164	GVDQIHIKPGAKVLY	ubiquitination	[2]
211	RDLINLAKKRTNIIP	acetylation	[3]
298	KMQQENMKPQEQLTL	acetylation	[4]
298	KMQQENMKPQEQLTL	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). 

Sequence Annotation

 REGION 178 179 S-adenosyl-L-methionine binding (By
 REGION 197 198 S-adenosyl-L-methionine binding (By
 REGION 222 223 S-adenosyl-L-methionine binding (By
 BINDING 149 149 S-adenosyl-L-methionine (By similarity).
 BINDING 242 242 S-adenosyl-L-methionine (By similarity).
 MOD_RES 8 8 Asymmetric dimethylarginine (By
 MOD_RES 15 15 Asymmetric dimethylarginine (By
 MOD_RES 21 21 Asymmetric dimethylarginine (By
 MOD_RES 24 24 Asymmetric dimethylarginine (By
 MOD_RES 28 28 Asymmetric dimethylarginine (By
 MOD_RES 31 31 Asymmetric dimethylarginine (By
 MOD_RES 130 130 Phosphoserine.  

Keyword

 Complete proteome; Methylation; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; RNA-binding; rRNA processing; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 327 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IDA:MGI.
 GO:0001651; C:dense fibrillar component; IEA:Compara.
 GO:0001652; C:granular component; IDA:MGI.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0030515; F:snoRNA binding; TAS:MGI.
 GO:0006364; P:rRNA processing; TAS:MGI.
 GO:0016074; P:snoRNA metabolic process; IMP:MGI.
 GO:0008033; P:tRNA processing; IEA:InterPro. 

Interpro

 IPR000692; Fibrillarin.
 IPR020813; Fibrillarin_CS. 

Pfam

 PF01269; Fibrillarin 

SMART

  

PROSITE

 PS00566; FIBRILLARIN 

PRINTS

 PR00052; FIBRILLARIN.