CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005892
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neuronal acetylcholine receptor subunit alpha-5 
Protein Synonyms/Alias
  
Gene Name
 CHRNA5 
Gene Synonyms/Alias
 NACHRA5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45SEPSSIAKHEDSLLKubiquitination[1]
52KHEDSLLKDLFQDYEubiquitination[1, 2, 3]
71PVEHLNDKIKIKFGLubiquitination[1]
107KQEWIDVKLRWNPDDubiquitination[1, 2]
119PDDYGGIKVIRVPSDubiquitination[1, 2, 3, 4, 5]
149RFEGTSTKTVIRYNGubiquitination[2, 3]
209LEDQDVDKRDFFDNGubiquitination[2]
227IVSATGSKGNRTDSCubiquitination[2]
354AMAPLVRKIFLHTLPubiquitination[1]
377VDRYFTQKEETESGSubiquitination[1, 2, 3, 6, 7]
387TESGSGPKSSRNTLEubiquitination[1, 2, 6, 7]
409YITRHIMKENDVREVubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. 
Sequence Annotation
 CARBOHYD 155 155 N-linked (GlcNAc...) (Potential).
 CARBOHYD 183 183 N-linked (GlcNAc...) (Potential).
 CARBOHYD 229 229 N-linked (GlcNAc...) (Potential).
 DISULFID 170 184 By similarity.
 DISULFID 234 235 Associated with receptor activation (By  
Keyword
 Cell junction; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Polymorphism; Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 468 AA 
Protein Sequence
MAARGSGPRA LRLLLLVQLV AGRCGLAGAA GGAQRGLSEP SSIAKHEDSL LKDLFQDYER 60
WVRPVEHLND KIKIKFGLAI SQLVDVDEKN QLMTTNVWLK QEWIDVKLRW NPDDYGGIKV 120
IRVPSDSVWT PDIVLFDNAD GRFEGTSTKT VIRYNGTVTW TPPANYKSSC TIDVTFFPFD 180
LQNCSMKFGS WTYDGSQVDI ILEDQDVDKR DFFDNGEWEI VSATGSKGNR TDSCCWYPYV 240
TYSFVIKRLP LFYTLFLIIP CIGLSFLTVL VFYLPSNEGE KICLCTSVLV SLTVFLLVIE 300
EIIPSSSKVI PLIGEYLVFT MIFVTLSIMV TVFAINIHHR SSSTHNAMAP LVRKIFLHTL 360
PKLLCMRSHV DRYFTQKEET ESGSGPKSSR NTLEAALDSI RYITRHIMKE NDVREVVEDW 420
KFIAQVLDRM FLWTFLFVSI VGSLGLFVPV IYKWANILIP VHIGNANK 468 
Gene Ontology
 GO:0005892; C:acetylcholine-gated channel complex; IC:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0015464; F:acetylcholine receptor activity; IDA:UniProtKB.
 GO:0004889; F:acetylcholine-activated cation-selective channel activity; NAS:UniProtKB.
 GO:0035095; P:behavioral response to nicotine; IMP:UniProtKB. 
Interpro
 IPR027361; Acetylcholine_rcpt_TM.
 IPR006202; Neur_chan_lig-bd.
 IPR006201; Neur_channel.
 IPR006029; Neurotrans-gated_channel_TM.
 IPR018000; Neurotransmitter_ion_chnl_CS.
 IPR002394; Nicotinic_acetylcholine_rcpt. 
Pfam
 PF02931; Neur_chan_LBD
 PF02932; Neur_chan_memb 
SMART
  
PROSITE
 PS00236; NEUROTR_ION_CHANNEL 
PRINTS
 PR00254; NICOTINICR.
 PR00252; NRIONCHANNEL.