UniProt Accession

 ITB2_HUMAN; P05107; B3KTS8; D3DSM1; Q16418; Q53HS5; Q9UD72 

Genbank Protein ID

 M15395; X64072; X64073; X64074; X64075; X64076; X64077; X64078; X64079; X64080; X64081; X64082; X64083; X63924; X63925; X63926; AK095992; AK222505; AL163300; CH471079; CH471079; CH471079; BC005861; Y00057; S81234 

Genbank Nucleotide ID

 AAA59490.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; CAA45427.1; BAG53190.1; BAD96225.1; CAB90553.1; EAX09381.1; EAX09382.1; EAX09385.1; AAH05861.1; CAA68266.1; AAB21404.1 

Protein Name

 Integrin beta-2 

Protein Synonyms/Alias

 Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD18 

Gene Name

 ITGB2 

Gene Synonyms/Alias

 CD18; MFI7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
29	SQECTKFKVSSCREC	ubiquitination	[1]
96	EDHNGGQKQLSPQKV	ubiquitination	[1]
148	DDLRNVKKLGGDLLR	ubiquitination	[1]
174	GFGSFVDKTVLPFVN	ubiquitination	[1]
194	LRNPCPNKEKECQPP	ubiquitination	[1]
196	NPCPNKEKECQPPFA	ubiquitination	[1]
294	HLEDNLYKRSNEFDY	ubiquitination	[1]
332	RMVKTYEKLTEIIPK	ubiquitination	[1]
362	LIKNAYNKLSSRVFL	ubiquitination	[1]
379	NALPDTLKVTYDSFC	ubiquitination	[1]
462	DRSLCHGKGFLECGI	ubiquitination	[1]
479	CDTGYIGKNCECQTQ	ubiquitination	[1]
561	RGLCFCGKCRCHPGF	ubiquitination	[1]
633	ISCAECLKFEKGPFG	ubiquitination	[1]
636	AECLKFEKGPFGKNC	ubiquitination	[1]
658	QLSNNPVKGRTCKER	ubiquitination	[1, 2]
744	RFEKEKLKSQWNNDN	ubiquitination	[1, 2, 3]
755	NNDNPLFKSATTTVM	ubiquitination	[1, 2]
765	TTTVMNPKFAES***	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. 

Sequence Annotation

 DOMAIN 124 363 VWFA.
 REPEAT 449 496 I.
 REPEAT 497 540 II.
 REPEAT 541 581 III.
 REPEAT 582 617 IV.
 REGION 449 617 Cysteine-rich tandem repeats.
 MOTIF 397 399 Cell attachment site (Potential).
 METAL 138 138 Calcium; via carbonyl oxygen.
 METAL 141 141 Calcium.
 METAL 142 142 Calcium.
 METAL 347 347 Calcium.
 MOD_RES 23 23 Pyrrolidone carboxylic acid.
 MOD_RES 745 745 Phosphoserine; by PKC.
 MOD_RES 756 756 Phosphoserine.
 MOD_RES 758 758 Phosphothreonine; by PKC; in vitro.
 MOD_RES 759 759 Phosphothreonine (Potential).
 MOD_RES 760 760 Phosphothreonine; by PKC/PRKCA; in vitro.
 CARBOHYD 50 50 N-linked (GlcNAc...).
 CARBOHYD 116 116 N-linked (GlcNAc...).
 CARBOHYD 212 212 N-linked (GlcNAc...).
 CARBOHYD 213 213 N-linked (GlcNAc...); atypical.
 CARBOHYD 215 215 N-linked (GlcNAc...); atypical.
 CARBOHYD 254 254 N-linked (GlcNAc...) (Potential).
 CARBOHYD 501 501 N-linked (GlcNAc...) (Potential).
 CARBOHYD 642 642 N-linked (GlcNAc...) (Potential).
 DISULFID 25 43
 DISULFID 33 447
 DISULFID 36 62
 DISULFID 46 73
 DISULFID 191 198
 DISULFID 246 286
 DISULFID 386 400
 DISULFID 420 445
 DISULFID 449 467
 DISULFID 459 470
 DISULFID 472 481
 DISULFID 483 514
 DISULFID 497 512
 DISULFID 506 517
 DISULFID 519 534
 DISULFID 536 559
 DISULFID 541 557
 DISULFID 549 562
 DISULFID 564 573
 DISULFID 575 598
 DISULFID 582 596
 DISULFID 590 601
 DISULFID 603 612
 DISULFID 615 618
 DISULFID 622 662
 DISULFID 628 647
 DISULFID 631 643
 DISULFID 670 695  

Keyword

 3D-structure; Cell adhesion; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Integrin; Membrane; Metal-binding; Phosphoprotein; Pyrrolidone carboxylic acid; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 769 AA 

Protein Sequence

Gene Ontology

 GO:0008305; C:integrin complex; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004872; F:receptor activity; IEA:InterPro.
 GO:0006915; P:apoptotic process; NAS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007267; P:cell-cell signaling; NAS:UniProtKB.
 GO:0007160; P:cell-matrix adhesion; IEA:InterPro.
 GO:0006954; P:inflammatory response; NAS:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007229; P:integrin-mediated signaling pathway; NAS:UniProtKB.
 GO:0007159; P:leukocyte cell-cell adhesion; IDA:UniProtKB.
 GO:0007275; P:multicellular organismal development; IEA:InterPro.
 GO:0030593; P:neutrophil chemotaxis; IDA:UniProtKB.
 GO:0008360; P:regulation of cell shape; NAS:UniProtKB.
 GO:0050730; P:regulation of peptidyl-tyrosine phosphorylation; IDA:UniProtKB.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome. 

Interpro

 IPR015812; Integrin_bsu.
 IPR015439; Integrin_bsu-2.
 IPR014836; Integrin_bsu_cyt_dom.
 IPR002369; Integrin_bsu_N.
 IPR012896; Integrin_bsu_tail.
 IPR003659; Plexin-like.
 IPR016201; Plexin-like_fold.
 IPR002035; VWF_A. 

Pfam

 PF08725; Integrin_b_cyt
 PF07965; Integrin_B_tail
 PF00362; Integrin_beta 

SMART

 SM00187; INB
 SM00423; PSI
 SM00327; VWA 

PROSITE

 PS00022; EGF_1
 PS01186; EGF_2
 PS00243; INTEGRIN_BETA
 PS50234; VWFA 

PRINTS

 PR01186; INTEGRINB.