UniProt Accession

 G3P2_YEAST; P00358; D6VWI4 

Genbank Protein ID

 X60157; V01301; X87611; Z49509; BK006943 

Genbank Nucleotide ID

 CAA42725.1; CAA24608.1; CAA60931.1; CAA89531.1; DAA08800.1 

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase 2 

Protein Synonyms/Alias

 GAPDH 2 

Gene Name

 TDH2 

Gene Synonyms/Alias

 GPD2; YJR009C; J1433 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
24	MRIALQRKNVEVVAL	acetylation	[1]
24	MRIALQRKNVEVVAL	ubiquitination	[2, 3]
46	DYSAYMFKYDSTHGR	ubiquitination	[3]
63	GEVSHDDKHIIVDGH	acetylation	[1]
63	GEVSHDDKHIIVDGH	ubiquitination	[2, 3]
71	HIIVDGHKIATFQER	acetylation	[1]
108	KELDTAQKHIDAGAK	acetylation	[1]
108	KELDTAQKHIDAGAK	ubiquitination	[3]
116	HIDAGAKKVVITAPS	ubiquitination	[3]
137	VMGVNEEKYTSDLKI	acetylation	[1]
137	VMGVNEEKYTSDLKI	ubiquitination	[3]
143	EKYTSDLKIVSNASC	acetylation	[1]
143	EKYTSDLKIVSNASC	ubiquitination	[3]
184	HSMTATQKTVDGPSH	acetylation	[1]
192	TVDGPSHKDWRGGRT	acetylation	[1]
192	TVDGPSHKDWRGGRT	ubiquitination	[3]
213	PSSTGAAKAVGKVLP	acetylation	[1]
213	PSSTGAAKAVGKVLP	ubiquitination	[3, 4]
217	GAAKAVGKVLPELQG	acetylation	[1]
217	GAAKAVGKVLPELQG	ubiquitination	[3]
225	VLPELQGKLTGMAFR	acetylation	[1]
225	VLPELQGKLTGMAFR	ubiquitination	[3]
246	SVVDLTVKLNKETTY	acetylation	[1]
246	SVVDLTVKLNKETTY	ubiquitination	[3]
249	DLTVKLNKETTYDEI	acetylation	[1]
249	DLTVKLNKETTYDEI	ubiquitination	[3, 4]
257	ETTYDEIKKVVKAAA	acetylation	[1]
257	ETTYDEIKKVVKAAA	ubiquitination	[3]
261	DEIKKVVKAAAEGKL	ubiquitination	[3]
267	VKAAAEGKLKGVLGY	acetylation	[1]
267	VKAAAEGKLKGVLGY	ubiquitination	[3]
307	QLSPKFVKLVSWYDN	ubiquitination	[3]
331	DLVEHVAKA******	acetylation	[1]
331	DLVEHVAKA******	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 12 NAD (By similarity).
 REGION 149 151 Glyceraldehyde 3-phosphate binding (By
 REGION 209 210 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 150 150 Nucleophile (By similarity).
 BINDING 33 33 NAD (By similarity).
 BINDING 78 78 NAD; via carbonyl oxygen (By similarity).
 BINDING 180 180 Glyceraldehyde 3-phosphate (By
 BINDING 232 232 Glyceraldehyde 3-phosphate (By
 BINDING 314 314 NAD (By similarity).
 MOD_RES 302 302 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0009277; C:fungal-type cell wall; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:SGD.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IMP:SGD.
 GO:0006094; P:gluconeogenesis; IEP:SGD.
 GO:0006096; P:glycolysis; IEP:SGD.
 GO:0072593; P:reactive oxygen species metabolic process; IMP:SGD. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.