CPLM-007455

Genbank Nucleotide ID

DNA-binding protein inhibitor ID-1

Protein Synonyms/Alias

Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1

Homo sapiens (Human)

Position	Peptide	Type	References
2	******MKVASGSTA	ubiquitination	[1]
20	AGPSCALKAGKTASG	ubiquitination	[1, 2, 3]
23	SCALKAGKTASGAGE	ubiquitination	[2, 4, 5]
77	NGCYSRLKELVPTLP	ubiquitination	[2, 3, 4, 5]
88	PTLPQNRKVSKVEIL	ubiquitination	[1]
91	PQNRKVSKVEILQHV	ubiquitination	[1, 2, 5]

[1] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

DOMAIN 53 105 bHLH.
MOTIF 98 111 Nuclear export signal (By similarity).

Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Nucleus; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IC:GDB.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
GO:0001525; P:angiogenesis; TAS:BHF-UCL.
GO:0006915; P:apoptotic process; IEA:Compara.
GO:0043534; P:blood vessel endothelial cell migration; TAS:BHF-UCL.
GO:0030509; P:BMP signaling pathway; IEA:Compara.
GO:0032963; P:collagen metabolic process; IEA:Compara.
GO:0001886; P:endothelial cell morphogenesis; IEA:Compara.
GO:0007507; P:heart development; IEA:Compara.
GO:0060425; P:lung morphogenesis; IEA:Compara.
GO:0060426; P:lung vasculature development; IEA:Compara.
GO:0043392; P:negative regulation of DNA binding; IEA:Compara.
GO:0045668; P:negative regulation of osteoblast differentiation; IEA:Compara.
GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:GDB.
GO:0010621; P:negative regulation of transcription by transcription factor localization; TAS:BHF-UCL.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
GO:0031648; P:protein destabilization; IEA:Compara.
GO:0045765; P:regulation of angiogenesis; IEA:Compara.
GO:0043408; P:regulation of MAPK cascade; IEA:Compara.
GO:0046677; P:response to antibiotic; IEA:Compara.
GO:0006351; P:transcription, DNA-dependent; IEA:Compara.
GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:BHF-UCL.

IPR011598; bHLH_dom.
IPR026052; DNA-bd_prot-inh.