UniProt Accession

 TRM5_HUMAN; Q32P41; B2RN19; Q9P2F4 

Genbank Protein ID

 AK293158; AL160236; CH471061; BC108284; BC136606; BC136607; AB037814 

Genbank Nucleotide ID

 BAG56703.1; EAW80792.1; AAI08285.1; AAI36607.1; AAI36608.1; BAA92631.1 

Protein Name

 tRNA (guanine(37)-N1)-methyltransferase 

Protein Synonyms/Alias

 M1G-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5 homolog 

Gene Name

 TRMT5 

Gene Synonyms/Alias

 KIAA1393; TRM5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
264	GEQNMMTKVRENNYT	ubiquitination	[1]
344	LYNCKLNKVDQKVKV	acetylation	[2]
348	KLNKVDQKVKVFNLD	acetylation	[2]
502	AEAFSDEKTQIVSNT	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. 

Sequence Annotation

 REGION 327 328 S-adenosyl-L-methionine binding (By
 REGION 355 356 S-adenosyl-L-methionine binding (By
 BINDING 289 289 S-adenosyl-L-methionine (By similarity).
 BINDING 387 387 S-adenosyl-L-methionine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Methyltransferase; Mitochondrion; Nucleus; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 509 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0052906; F:tRNA (guanine(37)-N(1))-methyltransferase activity; IEA:EC. 

Interpro

 IPR025792; tRNA_Gua_MeTrfase_euk.
 IPR003402; tRNA_Trfase_Trm5/Tyw2. 

Pfam

 PF02475; Met_10 

SMART

  

PROSITE

  

PRINTS